The antibody in question is "humanized" and described in a paper presenting clinical trial data
Hi, I am interested in recommendations of expression vectors for expression of proteins in E.coli and other Gram negative bacteria (potentially) that are independent of the T7 TNA polymerase; eg...
10 November 2018 3,845 0 View
Does anyone know if sodium azide is cytotoxic to mammalian cells (293, CHO, murine hybridomas) at 0.1-0.2% w/v? Buffers such as MACS buffer seem to include it but I would have thought this would...
03 April 2018 5,669 3 View
Any thoughts on what is the preferred method for glucose feed control in E.coli fed-batch? Both seem to have their merits, but quite difficult to tune pump PID settings to avoid sub-optimally...
31 December 2017 2,608 2 View
Can anyone tell me where i can source this strain? It is a tolRA knockout; hypervesiculating. I cannot find it in the normal collections and correspondence with two corresponding authors who have...
07 August 2017 8,792 1 View
Looking for a good quality antibody to probe tagged proteins produce in E.coli. My last experience with several antibodies showed very different performance from different vendors.
10 November 2015 6,600 4 View
Can anyone point me to some useful references on methods to assist in clarification of crude homogenates of animal tissue? Centrifugation in our case is only partly successful and our current...
03 April 2014 1,928 1 View
We have purified some of Fabs and would like to get some feedback on the best way to store them. Ideas on • Which buffer? • With/without glycerol? • 4oC or -20 oC? Would be greatly appreciated.
11 December 2013 3,001 2 View
Any suggestions of culture collections or other sources where we could obtain this culture?
09 October 2013 2,336 2 View
I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.
11 August 2024 9,101 4 View
I am developing a predictive model for a water supply network that involves 20 influencing points. However, I only have historical data for 10 out of these 20 points. I would like to know how to...
10 August 2024 4,005 2 View
I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...
10 August 2024 7,429 2 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
Request Python code from this article : Gender equity of authorship in pulmonary medicine over the past decade. THANKS!
08 August 2024 6,242 2 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
Visual Studio Code (VS Code) has become a popular choice among developers for several reasons: 1. **Free and Open Source**: VS Code is free to use and open source, making it accessible to...
07 August 2024 7,013 4 View
I received an e-mail invitation to join the editorial board of Clinical Cardiology Updates. While I have published a few articles related to cardiovascular disease, there are lots of colleagues...
06 August 2024 8,981 8 View
Hello all, I wanted to know, can I use galaxy (USA, Europe or Australia) platform for analyzing the shotgun data, and can it be used for publication purpose as well? Thanks :)
06 August 2024 6,610 4 View
Do experts have journals in the field of artificial intelligence and big data that are not indexed by SCI or EI?
05 August 2024 8,836 2 View