Broad host range vectors for protein expression in gram negative bacteria?

Hi, I am interested in recommendations of expression vectors for expression of proteins in E.coli and other Gram negative bacteria (potentially) that are independent of the T7 TNA polymerase; eg...

10 November 2018 3,779 0 View

Toxicity of sodium azide to cultured mammalian cells?

Does anyone know if sodium azide is cytotoxic to mammalian cells (293, CHO, murine hybridomas) at 0.1-0.2% w/v? Buffers such as MACS buffer seem to include it but I would have thought this would...

03 April 2018 5,620 3 View

PH-Stat or DO-Stat?

Any thoughts on what is the preferred method for glucose feed control in E.coli fed-batch? Both seem to have their merits, but quite difficult to tune pump PID settings to avoid sub-optimally...

31 December 2017 2,566 2 View

I am trying to find out where to ontain the E.coli strain JC8031

Can anyone tell me where i can source this strain? It is a tolRA knockout; hypervesiculating. I cannot find it in the normal collections and correspondence with two corresponding authors who have...

07 August 2017 8,734 1 View

We want to purchase an anti-HIS antibody for Western blotting. Any recommendations?

Looking for a good quality antibody to probe tagged proteins produce in E.coli. My last experience with several antibodies showed very different performance from different vendors.

10 November 2015 6,553 4 View

Can anyone suggest references to assist in the clarification of crude tissue homogenates?

Can anyone point me to some useful references on methods to assist in clarification of crude homogenates of animal tissue? Centrifugation in our case is only partly successful and our current...

03 April 2014 1,883 1 View

Storage of Fab antibody fragments?

11 December 2013 2,950 2 View

Does anyone know where can I obtain a culture of Candida antarctica?

Any suggestions of culture collections or other sources where we could obtain this culture?

09 October 2013 2,285 2 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature)?

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature-based reflection)? Thank you for your time and for...

03 March 2021 2,870 3 View

What degree of missing data in a particular case/participant results in Expectation-Maximisation no longer working?

I'm dealing with a mediation model and am using the PROCESS module in SPSS. Due to SPSS and PROCESS being limited in the imputation methods - being unable to handle multiple imputation - the other...

02 March 2021 4,362 1 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

How to code open-ended questions in SPSS?

Hello all, In SPSS I am going to code 2 open-ended questions. I have already read all the answers and I made a list of the most important categories to which I can code the answers. This question...

02 March 2021 1,757 4 View

Can NFL theorem be valid in infinite search space in ML?

NFL theorem is valid for algorithms training in fixed training set. However, the general characteristic of algorithms in expanded or open dataset has not been proved yet. Could you show your...

01 March 2021 1,189 3 View

Does anyone know how to plot my station to a section plot on Ocean Data View (ODV)?

I made a vertical section plot with WOA .nc file on ODV, and now I want to plot my stations (csv. file) in it. Does anyone know how to import my points to the section? They are two different...

01 March 2021 3,610 1 View