Broad host range vectors for protein expression in gram negative bacteria?

Hi, I am interested in recommendations of expression vectors for expression of proteins in E.coli and other Gram negative bacteria (potentially) that are independent of the T7 TNA polymerase; eg...

10 November 2018 3,779 0 View

Toxicity of sodium azide to cultured mammalian cells?

Does anyone know if sodium azide is cytotoxic to mammalian cells (293, CHO, murine hybridomas) at 0.1-0.2% w/v? Buffers such as MACS buffer seem to include it but I would have thought this would...

03 April 2018 5,620 3 View

PH-Stat or DO-Stat?

Any thoughts on what is the preferred method for glucose feed control in E.coli fed-batch? Both seem to have their merits, but quite difficult to tune pump PID settings to avoid sub-optimally...

31 December 2017 2,566 2 View

We want to purchase an anti-HIS antibody for Western blotting. Any recommendations?

Looking for a good quality antibody to probe tagged proteins produce in E.coli. My last experience with several antibodies showed very different performance from different vendors.

10 November 2015 6,553 4 View

In antibody nomenclature, what is meant by IgG2.delta.a?

The antibody in question is "humanized" and described in a paper presenting clinical trial data

04 May 2015 5,423 0 View

Can anyone suggest references to assist in the clarification of crude tissue homogenates?

Can anyone point me to some useful references on methods to assist in clarification of crude homogenates of animal tissue? Centrifugation in our case is only partly successful and our current...

03 April 2014 1,883 1 View

Storage of Fab antibody fragments?

11 December 2013 2,950 2 View

Does anyone know where can I obtain a culture of Candida antarctica?

Any suggestions of culture collections or other sources where we could obtain this culture?

09 October 2013 2,285 2 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to upload pdf of my articles published?

How to upload pdf of my articles published?

03 March 2021 2,183 3 View

Would adding books published in Lambert Publishers increase my RG impact?

There are some books published in LAP. Shall I add it to the RG?

02 March 2021 2,753 1 View

Is Journal of Agricultural Research a predator?

it is published by Juniper

28 February 2021 354 4 View

How can I publish a manuscript in free or low cost journal with good H-Index?

Dear all I am going to publish my manuscript which is from my thesis in a good H-Index journal but I found them very expensive(I don't have financial support) based on the exchange currency of my...

28 February 2021 7,800 6 View

Which software is better to write systematic review paper?

We have seen some authors use some software to write review paper. As obvious, the study of all published papers concerning our studying field is not possible and these software can help us to...

27 February 2021 6,296 1 View

Can I put a figure that was drawn by Bio-Render in publication paper?

I want to publish paper in journal but I have figures which have drawn with bio-renders, can I put them in the paper or which other software I can use to draw them?

27 February 2021 8,179 1 View

Can anyone please help me in processing SAR SLC data to generate interferogram using SNAP?

I have two scenes of the study area, one top and the other bottom. As per the tutorials and papers published, the step include coregistering the master sand slave images. However, when i try to...

24 February 2021 6,372 1 View

How do I change a preprint to published?

I want to know how to change the status of an article.

21 February 2021 1,536 2 View

How I can add publication with maiden name?

Good morning, can You please tell me how I can add an article that was published under my maiden name? When I try to add an article with this name, it appears information that I should be an...

21 February 2021 6,866 3 View