9 Questions 2 Answers 0 Followers
Questions related from Christopher P Marquis
Hi, I am interested in recommendations of expression vectors for expression of proteins in E.coli and other Gram negative bacteria (potentially) that are independent of the T7 TNA polymerase; eg...
11 November 2018 3,829 0 View
Does anyone know if sodium azide is cytotoxic to mammalian cells (293, CHO, murine hybridomas) at 0.1-0.2% w/v? Buffers such as MACS buffer seem to include it but I would have thought this would...
04 April 2018 5,656 3 View
Any thoughts on what is the preferred method for glucose feed control in E.coli fed-batch? Both seem to have their merits, but quite difficult to tune pump PID settings to avoid sub-optimally...
01 January 2018 2,597 2 View
Can anyone tell me where i can source this strain? It is a tolRA knockout; hypervesiculating. I cannot find it in the normal collections and correspondence with two corresponding authors who have...
08 August 2017 8,777 1 View
Looking for a good quality antibody to probe tagged proteins produce in E.coli. My last experience with several antibodies showed very different performance from different vendors.
11 November 2015 6,589 4 View
The antibody in question is "humanized" and described in a paper presenting clinical trial data
05 May 2015 5,458 0 View
Can anyone point me to some useful references on methods to assist in clarification of crude homogenates of animal tissue? Centrifugation in our case is only partly successful and our current...
04 April 2014 1,915 1 View
We have purified some of Fabs and would like to get some feedback on the best way to store them. Ideas on • Which buffer? • With/without glycerol? • 4oC or -20 oC? Would be greatly appreciated.
12 December 2013 2,984 2 View
Any suggestions of culture collections or other sources where we could obtain this culture?
10 October 2013 2,321 2 View
