28 July 2022 1 4K Report

could you help me in forming a circular DNA !

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How to fliter gravity waves from vertical wind shear and richardson number in the UTLS region?

I calculated vertical wind shear and gradient Richardson number using model output variables. however, i want to separate gravity wave signals from shear and Richardson number in the UTLS (upper...

04 July 2023 8,875 2 View

Salt water intrusion into the coastal aquifer of mombasa?

Problems associated methodology recommendations

11 April 2023 1,428 1 View

Suggestions to eliminate RNA contamination from human RNA binding protein expressed in E.coli?

I am expressing a human RNA binding protein in E. coli and purifying it using ammonium sulfate precipitation followed by heparin, butyl, and size exclusion chromatography. While the first batch of...

03 April 2023 6,022 3 View

How to design reverse primer with affinity tag, if stop codon is already present in the sequence?

I am trying to design a reverse primer with an affinity tag ( C-term affinity tag). Perhaps, my original protein sequence already has a stop codon at the C term. Could anyone suggest the correct...

15 January 2023 2,602 2 View

Does anyone know the composition of mitochondrial protein extraction buffer / mitochondrial lysis protocol other than sonitation or digitonin?

I am trying to screen the expression of mitochondrial protein from HEK293 cells. Unfortunately, Sonication is not recommended for my protein. Could anyone suggest a protocol for lysis?

05 December 2022 3,388 7 View

How to remove water from essential oil extract with the help of sodium sulphate?

Please provide the protocol for dehydration with sodium sulphate

31 August 2022 8,581 4 View

How to use Keithley 4200 to record charge and discharge of Zn Air battery ?

what parameters should be there? And how to set this parameters???

20 July 2022 5,550 0 View

How to calculate % of inhibition in antibacterial activity when the control shows no activity against the organism??

Antibacterial activity was performed against gram positive and gram negative bacteria using 0.05M HCl as control. in gram positive bacteria control has not shown any significant activity whereas...

17 March 2022 3,626 5 View

When photocatalytic degradation is carried out using various parameters . How should i calculate the calculate the rate constant and order ??

when pH,amount of catalyst and concentration of the dye are considered

15 January 2022 9,410 4 View

How could we rectify the fouling problem of milk in magnetic induction heating?

Fouling is one of the major problems in milk heating. What will be the probability of fouling of milk in a magnetic induction-based heating system? What are the precautions to be taken in order to...

29 October 2021 9,412 7 View

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I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

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Why does my protein refolded to beta sheet during thermal denaturation analysis?

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Why after performing site directed mutagenesis ,I don't see any colony after transformation?

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Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

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Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

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Are the apoptotic cells is positive for γH2AX ?

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Can anyone help me explain my problem?

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Illustra™ MicroSpin™ G-25 columns what it is used for?

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