To evaluate axon and neurite growth, which cells can be co-culture with Schwann cells in vitro?

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Malcolm Nobre

Hello Yasaman Ebrahimikia

You may use Neuro2a (N2a) cells with Schwann cells (SW10) besides PC-12. Please refer to the article attached below for more information.

Article Laser-Structured Si and PLGA Inhibit the Neuro2a Differentia...

You may also try NG108-15 neuronal cells with RN22 Schwann cells. You may refer to the article attached below for more information.

https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.737812

Best.

Noor Rajeeb

When co-culturing Schwann cells in vitro to evaluate axon and neurite growth, several cell types can be used as partners for co-culture experiments. Here are some cell types commonly used in co-culture systems with Schwann cells:

Dorsal root ganglion (DRG) neurons: DRG neurons are commonly used in co-culture systems with Schwann cells. They are primary sensory neurons that can extend axons and form neurites, making them suitable for studying axon growth and guidance.

Embryonic or adult hippocampal neurons: Hippocampal neurons are frequently used in neuronal culture studies. Co-culturing hippocampal neurons with Schwann cells can provide insights into axon development and plasticity.

Cerebellar granule neurons: Cerebellar granule neurons are another type of primary neurons that can be co-cultured with Schwann cells. They are often used to study axon growth and guidance mechanisms.

Cortical neurons: Cultured cortical neurons can also be co-cultured with Schwann cells to investigate axon and neurite outgrowth, as well as cellular interactions between different cell types.

Motor neurons: Motor neurons play a crucial role in controlling muscle movement. Co-culturing Schwann cells with motor neurons can provide insights into axon regeneration and motor neuron function.

Besides PC12 cells, which you mentioned, other cell lines like Neuro2A, SH-SY5Y, or N2A can be used for co-culture experiments. These cell lines are commonly employed in neurobiology research and can exhibit neurite outgrowth when co-cultured with Schwann cells.

It's important to select the most appropriate cell type based on the specific research objectives and the compatibility of the cell types being co-cultured.