Why some scientists do not believe in Climate Change?, and are based on the fact that in the Middle Ages, the temperature was higher?

01 February 2019 563 2 View

If I have part of the DNA, of a tyrannosaur and complete it with the DNA found from another tyrannosaur, will I be able to clone it?

If I have part of the DNA, of a tyrannosaur and complete it with the DNA found from another tyrannosaur, can we be able to clone it on the mother cells of a Crocodile?

31 December 2018 4,864 9 View

Until how long of an animal preserved in time, by cold or drought, can its DNA be obtained, to achieve its cloning?

If you have a part of an animal, that you can recover his DNA.

10 November 2017 4,031 2 View

What can be done with copper smelting slag?

I mean an environmentally friendly solution.

02 March 2017 4,952 5 View

Anyone knows if there is any scientific study of the impact on human health of the wireless signal?

I mean the WIFI signal for computers at home or at work.

03 April 2016 6,562 4 View

Could some plants not grow in height in 6 months or even shrink a little?

I'm thinking of my measures that I took every month on a plantation made in a tailing dam near to Copiapo city, in Chile. The species in question is the Schinus polygamus.

01 January 1970 195 5 View

Climate Change, it is really our fault?

Why do so many people pay so much attention to the end of the world that a girl preaches and they don't pay attention to what the Nobel prizes say, about the Climate Change?

01 January 1970 8,653 12 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Can linear DNA be used as template for site-directed mutagenesis?

Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...

03 March 2021 401 4 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Expression cloning by using cDNA,do I need to modify the cDNA first?

I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...

28 February 2021 5,440 3 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View