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I'm using 1 ml of Tetra Butyl ammonium hydroxide (20%) in my mobile phase(1 lit) to get good resolution. after 1 hr equilibration, if i run the Standard, the RT are found to be changing,...
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Is there any instrumental technique to find the number of water molecules present in an unknown compound?
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I'm using a 250x4.6 mm column on HPLC, and the RT of the analyte is 3.85 min.
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