Immunohistochemistry - How to do it?

Hi, I have got excellent results recently from my mice study on lung cancer xenograft tumors. I am looking to perform immunohistochemistry for analyzing the target proteins. We have never...

05 June 2019 9,095 4 View

Is 65% sequence identity good enough for basic modelling using Modeller?

I am trying to model a protein based on its homology. The two share 65% sequence identity. Is that good enough for basic modeling or should I opt for advanced modeling using Modeller? Btw, The...

02 March 2019 7,756 11 View

Can docking results from autodock using vina be published?

Hi, I have performed a docking study using my compound with a proteins obtained from pdb wesbite. I have used autodock vina for this. Can this results be published in a manuscript. It would be...

01 February 2019 1,276 10 View

Software for homology modelling?

I want to perform homology modelling for proteins from GLUT family. I have GLUT1 and GLUT3 (outward open conformation). Can someone suggest any software for generating homology modelled GLUT2...

01 February 2019 1,762 4 View

How to removes excess blocking?

I have been working with western blots and got great bands for actin. After that I stripped membrane with harsh stripping buffer but then blocked for 3hours (5% non-fat milk). I was not able to...

31 December 2018 3,296 7 View

Crispr ko cell line?

Hi, I generated a crispr ko cell line. The protein was more than 90% knocked out. But, now after several months od thawing and repassaging,the protein seems to comeback. I plan to do a grloth...

31 December 2018 9,505 10 View

How can I identify metabolites from LC-MS/MS data?

I just performed LC-MS/MS analysis. I have got a file with m/z, retention time, fragment score, etc. I want to know how can I do a pathway analysis and identify compounds? Which softwares...

11 December 2018 2,467 17 View

What is wrong with my transfer during western blot?

I tried to transfer today and found that the membrane (membrane 1) looked like this after ponceau S staining. Two days ago, I transferred two membranes together in the same cassette and found...

11 December 2018 2,545 7 View

Is my blot publishable?

Hi, I am trying to blot a protein called GLUT1. It is transmembane protein known for glycosylation. So I expected to observe streak rathen than a single band. This is what I got. I tried to KO...

08 September 2018 8,787 1 View

Doxorubicin to induce nercosis?

I am working on A549 cells with an anticancer compound. I am planning to study the mechanism of cell death in those cells (apoptosis or necrosis). Since, I am using flow cytometry, I want to use...

05 June 2018 7,327 3 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View