Hi everyone, I have a question, I am working with a very low dose of malathion (Non-cholinergic dose) and long-term exposure 48hrs toxicity in vitro. I got the IC50 value at 48hrs. So, Could you please suggest to me how I go for further experiments like ROS, MMP, Cell Cycle Arrest, and Apoptosis. I am confused about the time point related to these experiments. After this experiment. I want to do prevention studies. Please give me some guidance, suggestion, and information.

Thank you