I am studying a Acyl carrier Protein in Pseudomonas aeruginosa.
C- and H-NMR for example will give you data that allow you to know exactly how long is your acyl chain ;). I've done it with marine siderophores, worked like a charm. Good luck!
Hi, everybody, I am now working on the localization of flagellum in Pseudomonas aeruginosa. I need to construct a fluorescence fusion protein. It is better to have CFP or YFP in a shuttle vector....
09 October 2015 2,482 2 View
I am trying to see the sub-location of my protein. I use E.coli-Pseudomonas shuttle vector, pUCP20, to construct GFP-protein fusion plasmid, which could be induced by1 mM IPTG. When I used Leica...
06 July 2015 9,083 4 View
I need to construct a conditional mutant for an essential gene in PA14. At first, I knocked in a copy under pBAD promoter into chromosome via attB site using integration plasmid. Gene expression...
03 April 2015 8,394 11 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...
06 August 2024 1,989 3 View
Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...
04 August 2024 8,894 1 View
Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC
01 August 2024 967 3 View
Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...
31 July 2024 9,892 4 View
Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....
29 July 2024 5,007 0 View
I want to do 2,3-butanediol dehydrogenase(BDH) enzyme purification to confirm its activity for 2,3-butanediol. Before that, I need to confirm which N or C terminal tagging is better for enzyme...
28 July 2024 366 3 View
I cannot confirm cell surface protein expression by flow cytometry even after transfection and antibiotic selection of cells. Does it take long for proteins to express on the cell surface? the...
28 July 2024 3,178 3 View
I'm having problems with ion exchange chromatography. After applying my sample (a fluorescent protein), a preferential path inevitably forms. The matrix I use is DEAE-Sephacel. Could someone...
27 July 2024 6,605 0 View
It has been long known that EDC carbodiimide and NHS easter's half life agains hydrolysis are highly related to pH. However, very surprisingly, not yet found a paper giving a table/chart of their...
25 July 2024 8,738 0 View