Hi, everybody,
I am now working on the localization of flagellum in Pseudomonas aeruginosa. I need to construct a fluorescence fusion protein. It is better to have CFP or YFP in a shuttle vector. I appreciate it if anyone could help.
Mitja Remus-Emsermann
Check out addgene.com you can order many plasmids (including plasmids suitable for the construction of fusion proteins) for a very reasonable price over there (± 65 $ if I am not mistaken)
Most E.coli plasmids should function just fine in P.a.
Full disclosure: No commercial interest from my side :)
Cheers and good luck
1 votes
1 thanks
Marie Erard
I would advise you to use Aquamarine or mTurquoise instead of CFP. They are both brighter, more photostable and insensitive to their environment,
marie
1 votes
1 thanks
- Badges
- Science topic
More Wei Chen's questions See All
Similar questions and discussions