Wei Chen

4 Questions 8 Answers 0 Followers

Questions related from Wei Chen

Does anybody have CFP or YFP fusion plasmid I can request?

Hi, everybody, I am now working on the localization of flagellum in Pseudomonas aeruginosa. I need to construct a fluorescence fusion protein. It is better to have CFP or YFP in a shuttle vector....

10 October 2015 2,460 2 View

Does anybody use GFP fusion to observe protein location in Pseudomonas aeruginosa?

I am trying to see the sub-location of my protein. I use E.coli-Pseudomonas shuttle vector, pUCP20, to construct GFP-protein fusion plasmid, which could be induced by1 mM IPTG. When I used Leica...

07 July 2015 9,062 4 View

Can anybody give some suggestions for knocking out essential genes in Pseudomonas aeruginosa?

I need to construct a conditional mutant for an essential gene in PA14. At first, I knocked in a copy under pBAD promoter into chromosome via attB site using integration plasmid. Gene expression...

04 April 2015 8,373 11 View

There is an acyl chain binding with my protein. How can I identify this flanking chain?

I am studying a Acyl carrier Protein in Pseudomonas aeruginosa.

11 November 2012 162 1 View