Can you suggest some plant transformation vectors that I can use for the expression of GFP/YFP-tagged protein of my interest?

I want to generate stable Arabidopsis transgenic lines expressing my protein of interest fused with GFP/YFP under the control of its native promoter. I want this for localization studies. Please...

23 January 2021 2,790 3 View

Precipitation of Pcl from polymeric solution ?

We working on PCL nano particles 1).Ketoprofen (10mg)+ dmso(5ml) Pcl (10mg)+ DCM(5ml) 2).Water 10 ml+ 10 mg When we mix both 1+2 after solublization (400 rpm) Why precipitation of polymer occurs?

09 December 2020 5,033 11 View

Can anyone suggest me an industry working in Biostimulants ?

it would be very helpful if anyone would suggest industries working in biostimulants.

25 October 2020 5,824 2 View

I am getting very high value for permeability through membranes?

i am working on gas separation based on flat sheet membranes. i was trying to measure the n2 permeability through the membranes and the value i am getting is very high. and the flow rate is also...

12 September 2020 8,632 11 View

How to calculate the permeability in barrer units??

HI, i am working gas separation using flat sheet membranes and I am new to this topic. I tried to find the permeability of nitrogen in the membrane by varying the inlet pressure and measuring the...

06 September 2020 4,656 3 View

HOw to conduct Battery sizing for Wind Farms and PV Farms?

Considering a battery for power output smoothing, how is it possible to calculate the capacity of a battery that can be used in a wind farm and a pv farm?

19 July 2020 5,213 1 View

I am interested in locating nutrients essential for photosynthesis. How can I see the chloroplast of lemongrass leaf using SEM ?

I am interested in locating nutrients important for photosynthesis. How can I locate them in lemongrass leaf using SEM or some other protocol.

07 July 2020 6,779 3 View

I am interested in locating nutrients essential for photosynthesis. How can I see the chloroplast of lemongrass leaf using SEM ?

I am interested in locating nutrients important for photosynthesis. How can I locate them in lemongrass leaf using SEM or some other protocol.

07 July 2020 2,194 3 View

How do we extract data from netcdf file that overlaps with shapely Polygon geometry in python?

I have data for precipitation in netcdf file format and a shapely polygon geometry. I want to extract only the data that overlaps with the polygon. Any idea how we do that in python?

01 March 2020 9,938 2 View

How do we do a linear regression on estimated trend in time series data?

How do we do a linear regression on estimated trend in time series data? What is meant by trend prediction = time +time*time+error?

11 November 2019 4,620 3 View

How do you write a simplified C block code of this average-current control on PSIM?

This is a Buck (step-down) Converter and I am new to this program. I need to program with simplified C block and I actually don't know what to do. Can anyone show me how to do this? Or at least...

02 March 2021 8,233 1 View

Do you know good and user-friendly programs to measure plant quantitative traits?

For my research i will need to measure plant quantitative traits (especially leaves area and roots length, but would be nice to add some more information). I recently discovered...

01 March 2021 5,035 2 View

Is it possible to set a breakpoint at a specific DYNAMIC instruction using the GNU Debugger (GDB)?

Using GDB, it is straightforward to debug and monitor a target program by setting a break-point at a specific instruction, since the instruction addresses are known. However, we have two...

24 February 2021 1,598 2 View

Implementation of chance-constrained optimization problem via scenario approach?

23 February 2021 7,826 2 View

I am looking for a simple software program to model 3D chemical bonds. The purpose is to generate high-quality graphical abstracts. Any suggestion?

Hello everyone: I am looking for a simple software program to model chemical bonds. The purpose is to generate a high-quality graphical abstract for a top leading journal in the field of...

23 February 2021 8,012 3 View

Can I use FLEXBAR to detect and remove barcodes out of my short reads without demultiplexing ?

Hello, I have demultiplexed my short reads and I have removed the Illumina adapter left in the sequences and now I need to remove the barcodes used by the sequencing centre from my reads. I have...

22 February 2021 9,873 3 View

Any interests in participating to an experiment in space?

Hello, The UN Office of Outer Space Affairs has a program called access to space for all that enables institutions to have hands on expertise related to access to space. There are three elements...

22 February 2021 7,980 3 View

Simulation or Experiment? How do we choose?

If we want to assess if a phenomenon is going in a direction we expect, we can simulate it, using digital models, possibly agent models factoring in known properties and interaction features. We...

12 February 2021 1,141 21 View

Vth increase with programming SONOS Flash in Tcad?

Hello, I've been working on this issue for more than a month now.... I made a basic SONOS flash structure using TCAD Sentaurus sProcess. (tunnel oxide/nitride/blocking oxide : 4/6/6 [nm]) Then,...

11 February 2021 3,337 3 View

How to identify when a gaussian program is about terminate normally?

Dear all, I am trying to optimize a bimetallic complex using # opt=tight b3lyp/lanl2dz scf=qc geom=connectivity. The program is running for around 5 days but the size of the .chk remains same...

05 February 2021 8,721 3 View