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Does anyone has the profile of E coli. ATPase complex (F1Fo) size-exclusion chromatography (using a Superose 6 column), I mean what the profile looks like, how many peaks? Are they all well separated?
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In particularly，I don't know how to draw a 150-rows reteclar over a TRF lane and how to measure the ODi of each rows, and I'm wondering It has to be done one by one manually or there are some sort...
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