ELISA detection cytokine in human serum (common dilution fold)?

Hi everyone, I'm gonna try to detect the IL-10, IFN-y, TGF-beta1 level in the human serum by ELISA. I bought the ELISA set from the BD contain Capture Ab, Detection Ab, Enzyme reagent, Standards....

09 December 2020 3,369 5 View

Error when running both DEM and Bio file in Maxent?

Hello everyone, I am Trang. I have problem when used the Maxent to predict the potential distribution of some rare speices in Vietnam. I have 2 file *.ascii, including DEM and bio9. Althought all...

20 July 2020 8,440 4 View

Dopiing in semiconductor materials?

I'm a student in materials technology. Currently, my work is about codoping ZnS with Mn and Cu. However, i have some problem with the dopant substitute. I mean, how to determine which place that...

03 February 2020 1,865 7 View

Which should I choose: Pooled OLS, FEM or REM?

I am building panel data econometric models. Both the F-test and Breusch-Pagan Lagrangian test have statistical meaning, that is, the Pooled OLS is worse than the others. However, when testing...

08 September 2018 4,571 7 View

What's the PCR conditions for RPB1-F and RPB1-R primers?

13 July 2018 1,736 5 View

How to input and characterize live oil composition in HYSYS?

Please help me with this case. I have composition report up to C36+ of live oil from PVT lab and crude oil assay from the same sample. My problem is how to input these data in HYSYS. I can input...

21 March 2018 620 3 View

What is a suitable method for calculating prior probability in Bayesian classifier?

In Bayesian approach, the prior probability is often computed by the ratio of sample in training set. Is it really suitable? Is there any method to calculate the prior probability without...

02 March 2016 2,299 3 View

What is the acceptable value for chi-square goodness of fit in Electrochemical Impedance Spectroscopy

17 February 2016 8,879 11 View

The DNA concentration of Flag-C1 (control) and Flag-VAV2 should be different when transfect into the cell?

I am doing western blot to detect the activation of Rac1 (Rac1-GTP). VAV2 is known to activate Rac1. I want to confirm by western blot. The length of Flag-C1 (control) is shorter than Flag-VAV2...

14 January 2016 9,968 1 View

Can I compare the expression of two genes only based on their Ct value?

Dear all, I want to know the expression of target gene in tumor tissue but I don't have normal tissue to control. So can I compare target gene' Ct value with Ct of GAPDH? If the mean of target...

23 August 2015 6,818 29 View

Bayesian decision trees and bayesian regression trees in Python and/or R?

I am looking for implementation of bayesian decision trees and bayesian regression trees in Python and/or R.. Do you know if the existing libraries support this

23 February 2021 6,689 3 View

Can't recognize exact phase and PDF card no of Silicon carbide in XRD?

We have deposited silicon carbide on carbon substrate using one PVD technique. Due to amorphous behavior of SiC at low deposition temperature, we performed heat treatment of those films at...

22 February 2021 2,072 3 View

What cause the beating of this signal?

I am trying to simulate the impact of a sinusoidal force on a Beam, yet I registred this beating signal (Displacement by time). The freaquency of excitation was the same as the first natural...

19 February 2021 5,872 3 View

What is the minimum number of studies in each category required for a multiple subgroup analysis to be viable in a meta-analysis?

Cochrane Handbook mentions subgroup analyses are only warranted if there are more than 10 studies (I have 28), and if they are distributed evenly among categories of subgroup. I am wondering if...

17 February 2021 3,467 4 View

Access to supercomputer resources in India?

Hello everyone, I want to perform some gaussian calculations and want to use Materials studio for my research work. Can anyone guide me how to get access to supercomputer resources in India for...

15 February 2021 8,264 3 View

Which Journals would you advise to submit a Letter which briefly talks about neurosurgical treatment of neuro-oncological patient during Sars-COV-2?

Possibily not an open access journal which does not add any publishing costs once being accepted. Thank you in advance

09 February 2021 717 6 View

How to do mono formylation of N,N-diethyl-3-methoxybenzamide?

I have attempted the formylation of the above mentioned compound using a very well reported protocol using secondary Butyl Lithium and TMEDA at - 78 degrees Celsius. I have done it quite a few...

08 February 2021 2,793 2 View

I am looking for a co-author specialised in decision-making/managerial studies/data science/?

I am currently working on a publication with the intention of presenting a proprietal method for a ranked comparison of incompatible features in a limited data set. I am looking for a co-author as...

08 February 2021 1,450 5 View

What are the limits of determining metastability in the brain?

What are temporal limits in determining brain metastability? In other words, how long does the minimum length of the scan segment using fMRI have to be for the result to be of any value? I'm...

07 February 2021 2,380 2 View

Does Abaqus plugin AM-Modeler predict the same temperature/crystallinity history as UMATHT user-subroutine?

During AM process, progressively activation of elements can predict the temperature/crystallinity history, including surroundings heat losses and than this data could be used to determine the...

06 February 2021 4,686 1 View