5'ppp-dsRNA transfection to stimulate RIG-I using Lipofectamine 2000 protocol?

18 May 2023 1 7K Report

Hi everyone,

I need to transfect 5'ppp-dsRNA (invivoGen) to stimulate RIG-I pathway using Lipofectamine 2000 for Bone marrow-derived macrophage.

If you have experience with this, please share your protocol.

The company manual only mentions about DNA transfection or siRNA transfection, however, I need specific protocol for 5'ppp-dsRNA.

Thank you so much.

Eric Perouzel

You can use the same ratio as recommended by the manufacturer for siRNA. At the end of the day it is a matter of phosphodiester charged versus cationic lipids...so you should be OK. Best

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