ELISA detection cytokine in human serum (common dilution fold)?

Hi everyone, I'm gonna try to detect the IL-10, IFN-y, TGF-beta1 level in the human serum by ELISA. I bought the ELISA set from the BD contain Capture Ab, Detection Ab, Enzyme reagent, Standards....

09 December 2020 3,369 5 View

Error when running both DEM and Bio file in Maxent?

Hello everyone, I am Trang. I have problem when used the Maxent to predict the potential distribution of some rare speices in Vietnam. I have 2 file *.ascii, including DEM and bio9. Althought all...

20 July 2020 8,440 4 View

Dopiing in semiconductor materials?

I'm a student in materials technology. Currently, my work is about codoping ZnS with Mn and Cu. However, i have some problem with the dopant substitute. I mean, how to determine which place that...

03 February 2020 1,865 7 View

Which should I choose: Pooled OLS, FEM or REM?

I am building panel data econometric models. Both the F-test and Breusch-Pagan Lagrangian test have statistical meaning, that is, the Pooled OLS is worse than the others. However, when testing...

08 September 2018 4,571 7 View

What's the PCR conditions for RPB1-F and RPB1-R primers?

13 July 2018 1,736 5 View

How to input and characterize live oil composition in HYSYS?

Please help me with this case. I have composition report up to C36+ of live oil from PVT lab and crude oil assay from the same sample. My problem is how to input these data in HYSYS. I can input...

21 March 2018 620 3 View

The process for an article be accepted?

My article's status has been "decision in process" for one month but I am not received the email of editor? What's happen with my article?

02 March 2016 5,729 2 View

What is a suitable method for calculating prior probability in Bayesian classifier?

In Bayesian approach, the prior probability is often computed by the ratio of sample in training set. Is it really suitable? Is there any method to calculate the prior probability without...

02 March 2016 2,299 3 View

What is the acceptable value for chi-square goodness of fit in Electrochemical Impedance Spectroscopy

17 February 2016 8,879 11 View

The DNA concentration of Flag-C1 (control) and Flag-VAV2 should be different when transfect into the cell?

I am doing western blot to detect the activation of Rac1 (Rac1-GTP). VAV2 is known to activate Rac1. I want to confirm by western blot. The length of Flag-C1 (control) is shorter than Flag-VAV2...

14 January 2016 9,968 1 View

Dear all, I was wondering if anyone knows whether MGLtools and Autodock can run on Mac OS?

I have tried to download the 64-bit version of MGLtools however, as many times as I have downloaded and uninstall the programs, an error arises that the app needs to be updated. Thank you.

03 March 2021 5,997 2 View

I got 02 moderator values defining Johnson-Neyman significance regions. Is it an error?

Hi everyone, I am conducting research for my Master's thesis. I am using PROCESS by Johnson-Neyman to analyze my Moderator model. I test the relationship between Public Service Motivation and...

03 March 2021 2,350 2 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Do I need to strip the PVDF membrane before staining it with colloidal gold?

Dear Colleagues, After running Western blot on PVDF membrane and detection using ECL, I would like to stain my PVDF with colloidal gold to be able to allign the ECL image with total proteins on...

02 March 2021 7,829 3 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

OpenBCI eeg--has anyone used them?

I'm looking to try out an off-the-shelf EEG set for some exploratory work. Curious if anyone has used the OpenBCI, and how much set-up work is required. I've heard they are more difficult to get...

01 March 2021 4,128 3 View

Is there any expert in Smart PLS who can help me?

Dear colleagues, I have a manuscript that needs an expert in smart PLS to run the analysis, Is there anyone expert and ready to help me, please?

01 March 2021 8,212 3 View

Why initial concentration of Pb not the same as calculated one when testing using AAS?

I am currently doing research on the adsorption of heavy metal using magnetic reduce graphene oxide. I use AAS to determine the concentration of the heavy metal. I prepare the Pb solution with...

26 February 2021 9,580 4 View

How to run Cross-lagged panel Design on AMOS?

25 February 2021 3,126 1 View