Hi everyone, I am performing soft agar colony formation assay on Jurkat and activated T cells. The agar concentration are: 0.6% (top) and 0.3% (bottom). For both cell types, I am titrating different seeding cell densities. However, after 1 week, I noticed that Jurkat seems to be dying off, whereas human T cells viability was good.
Does any faced similar issue with cancer cell dying in soft agar assay? And how important it is that cancer cells must be in active proliferating phase when seeded for this assay?
thanks so much
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