During SDS-PAGE separation the sample buffer running faster than sample. Can anyone help me to fix this problem?
It looks like the dye in the ladder is running faster than the dye in the sample buffer. It may be a different dye, in which case there is no problem.
Hi,
its possible to have situation like this,the difference in the migration of your ladder dye front and sample as seen in your gel is not a problem because of difference in MW of the protein components in each sample and your marker.
I hope this will help you.
I agree with the above answers. The dye in your samples is probably different to the dye in the molecular weight marker. Once your protein is still expected between bands visible for the marker you shouldn't have any issues. For peice of mind, check the manufacturer of the sample loading dye and the marker to see if they are the same.
Dear all!
Thank you for your suggestions.
Our problem is that the dye (sample buffer) is separated from samples. Consequently, this front line don't shows correctly the separation.
Thank you!
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