The leishmania I am culturing just keeps dying off. Is there any way to revive them when they have already become too weak?

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Syed A Ali

I understand you are referring to promastigotes. Please inoculate them in NNN medium.

Francehuli Dagger

It is important to know the Leishmania strain . . Use culture flask with a thin layer of medium . ! It helps . Increase the amount of FBS

Chaoqun Yao

Leishmania spp. culturing is species, or even strain dependent. Try to use similar medium in literature for the species of your interest.

David Forrest

Hi Alberta, What species are you culturing? The link below is a pretty good guide written for culturing and manipulating leishmania

http://www.gla.ac.uk/media/media_91939_en.pdf

Mebrahtu Tedla

Try to keep the PH of your medium at 7.2 if you are reviving L.major promastigotes. Ideal Incubation temperature is 27 degrees.