Does anybody have experience in complete lost of AF647 signal in multicolor flow? any biochemical explanation?
I use a 3 laser (blue; red and violet (488; 635; 405 nm)
In particular I am talking about staining of CCR6 on human CD4 T cells.
At first please see the attachment. This experiment was performed for a period of 7 days (n=100). After 7 days, we found different level of survival rate (%) for each treatment. Now, I want to...
15 February 2021 9,145 2 View
The organo montmorillonite nanoclays modified with octadecylamine were used for the experiment. After acid treatment, the surface area will be increase. However, in my experiment, the surface area...
10 February 2021 1,581 3 View
Dear all, I am trying to optimize a bimetallic complex using # opt=tight b3lyp/lanl2dz scf=qc geom=connectivity. The program is running for around 5 days but the size of the .chk remains same...
05 February 2021 8,721 3 View
The recommended amount of DNA for restriction digestion is 1ug in a 50ul rx I've got around 500-600ng of DNA in a total volume of 35ul. Would I get satisfactory results with this amount of DNA for...
31 January 2021 729 6 View
Please share your experience on the effectiveness or challenges on online teaching during Covid-19 lock down. Share your experience about challenges, technology, online learning platform,...
26 January 2021 4,532 37 View
Recently I have generated LC-MS metabolomics data. After annotation, some have their compound name but either no KEGG id or KEGG id but no pathway. How to interpret those compounds? Additionally,...
18 January 2021 1,938 3 View
The current global pandemic, the climate crisis, the destruction of the oceans (with plastics, etc), and massive numbers of refugees are part of a set of serious global problems. It is clear...
13 January 2021 2,022 3 View
I want to remove a duplicate citation
01 January 2021 3,983 3 View
Hello, I am a newbie in geant4. I'm learning it playing with example. Sometimes, when I go to simulate an example code, it doesn't create an executable file (the green one in my ubuntu terminal)....
26 December 2020 9,482 3 View
Hello all, I want to do glycosaminoglycan (GAG) simulation in Gromacs. Initial structure and CHARMM topology for short chains can be obtained from Charmm GUI glycan modeler. But I have to take...
23 December 2020 6,092 2 View
Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...
03 March 2021 8,796 3 View
03 March 2021 5,360 2 View
Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...
01 March 2021 8,235 2 View
We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...
01 March 2021 4,937 1 View
How do I run a a priori sample size determination for non-parametric tests, like the Mann-Whitney U-test and the Kruskall-Wallis or other non-parametric tests that accord with the unpaired t-test...
24 February 2021 166 6 View
Hello, I will start performing MLR using lymphocytes from mice's spleen and I would like to know the better way for the stimulation. If is using PHA-P or CD3/CD28? I would like to know as well if...
23 February 2021 5,476 4 View
Hello Everyone, I have one population, sample size 200. and needed to find the correlation between Variable A and B. Also, which T-test should I choose for hypothesis testing Please advise...
21 February 2021 7,591 4 View
Hi, I am staining a human blood with CD19, CD3,CD14 and CD16. My gating strategy in FlowJo goes like this: - First, I gate a general population SSC-A VS FSC-A => Then gate for single cells, =>...
21 February 2021 5,724 4 View
This might sound very basic question, but please share with me an explanation of how the T cell is activated when the antigen is not a peptide? What happens? And what are the molecules involved in...
21 February 2021 564 3 View
I have two groups (unpaired) of plant biomass measurements and want to know whether there is a test that can be used to determine statistical significance where assumptions are not violated. The...
18 February 2021 1,258 4 View