Any insight is greatly appreciated. I am not sure if HBS and HBSS are the same.
Dear Alberta,
Here is the hbss composition with 25 mM Hepes.
https://www.aatbio.com/resources/buffer-preparations-and-recipes/hhbs-hanks-buffer-with-hepes
Similarly, you can find composition of PBS and other types of HBSS. http://cshprotocols.cshlp.org/content/2006/1/pdb.rec548.full
To me main difference between PBS and HBSS occur that later contain glucose.
Hepes is generally used to maintain hbss pH longer if it include cells out side incubator, for example, during microscopic examination of live cells.
Regards
Dear Alberta Kovatcheva,
Difference between these solution is the ingredients. Please see the following preparation-
1. Preparation of HEPES buffer saline-
Add 2.38 g of HEPES to a beaker. And add about 80 mL of deionized water to the beaker. Add a stir bar to the beaker and leave it on a stir plate until completely dissolved (Aprox.1 min). See the pH of solution.Adjust the pH 7.4 by adding the diluted solution of NaOH.
2. Preparation of HBSS-
3.Preparation of phosphate buffer saline
Take 800 ml of distilled water and add 8 g of NaCl and add 0.2 g of KCl and add 1.44 g of Na2HPO4 and add 0.24 g of KH2PO4.Adjust the pH to 7.4 with HCl. Finally add distilled water to a total volume of 1 liter.
Best of Luck.
Dear Alberta,
Following ingredient differences are found in the said buffers.
1. Preparation of PBS buffer (Dissolve 8 gm NaCl, 0.2 gm KCl, 1.15 gm Na2HPO4, 0.2 gm KH2PO4 in distilled water and make final volume 1000 ml).
2. Preparation of Hanks Balanced Salt Solution HBSS (Dissolve 8 g of NaCl, 400 mg KCl, 140 mg CaCl2, 100 mg MgSO4-7H2O, 100 mg MgCl2-6H2O, 60 mg Na2HPO4-2H2O, 60 mg KH2PO4, 1 g of Glucose, 350 mg NaHCO3 in distilled water and make final volume 1000 ml).
3. Preparation of HEPES-buffered saline (HBS) (Dissolve 2.38 g of HEPES in about about 80 mL of distilled water).
I can also contribute with the following, it depends for the use, for instances, you can use Hank´s sol. to avoid damage in the membrane of the cell, my work colleagues in electophysiology have used this solution without Ca++ and Mg++mintead of trypsin to detache the monolayer cells, and it works well. I have also confirmed it in analysis by flow cytometry, there are less dead cells that those handled with trypsin.
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