Hello, I want to add ellagic acid to the medium for fermentation transformation. According to the literature, I first dissolve ellagic acid in DMSO, and then add them to the medium together (ellagic acid: 20ppm , DMSO: 1/50), but after sterilization at 120°C for 15 minutes, flocculent substances appeared at the bottom of the medium. Is there any good way to add ellagic acid to the medium? Thank you.
Dear Zmw Zhang,
It is necessary to sterilize the medium without adding ellagic acid (EA). The EA solution must be filtered–sterilized through nylon membranes of 25-mm diameter and 0.45-μm pore size. Then as soon as the temperature of the sterilized medium reach to 30°C, the EA solution could be added to the medium.
Check https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3539995/
Also check https://onlinelibrary.wiley.com/doi/10.1111/1750-3841.14369
Thank you for your answer. I tried this method, but as time went on, the medium produced a little flocculation (although the flocculation was not as much as the previous method)@Mina-Karimi-Avargani
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