Sylvester Peter Antai
Gram positive bacteria retain the primary stain in their cell walls....Conversely, bacteria that decolorize, or give up the crystal violet - iodine complex will accept safranin counterstain and appear red. They are Gram negative ( G- ) bacteria. When over - decolorize by either prolonged exposure to decolorizeser or using acetone alone. When cell wall gets damaged by exposure to lysosomes or cell wall acting antibiotics such as Penicillin. Old cultures where the is cell wall is weaken or action of autolytic enzymes. Those bacteria that are phygocytosed. Where cell wall is acted upon by lysosomal contents. For more details consult https://www.quora.com
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Abdelazeem Algammal
When over-decolourized by either prolonged exposure to decolourizer or using acetone alone. When cell wall gets damaged by exposure to lysozyme or cell wall acting antibiotics such as Penicillin. Old cultures, where cell wall is weakened or action of autolytic enzymes Those bacteria that are phagocytosed. where cell wall is acted upon by lysosomal contents
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