I have frozen human tissue samples and I need to measure ADP/ATP ratio in these tissues using a bioluminescence kit from Abcam. what is the best method to prepare my samples without degrading ADP or ATP?
I did qualitative assay on agar plates with starch as sole carbon source for checking amylase activity, once I added iodine the clear zone appeared arround the colonies but after some hours and...
13 May 2024 6,529 2 View
I'm working with recombinant protein, it's a protease enzyme that we use E. coli to synthesize, and usually, it's stable for up to six months in the -80 freezer. Recently, the protein activity has...
17 April 2024 4,262 3 View
I am a graduate student doing sub cloning of two similar genes into pBC SK(+); one of the gene was inserted but few colonies appeared on the agar after transformation, the other is not inserting...
22 February 2024 7,237 5 View
In light of recent research findings and advancements in communication strategies for individuals with hearing loss, how can audiologists enhance patient-centered care in their practice? Are there...
17 December 2023 2,894 1 View
I have a four-story steel structure When conducting a pushover analysis and examining the results, the structure's ductility ratio at the performance point did not exceed 1, and the equivalent...
08 November 2023 934 4 View
I'm preparing Cadmium sulfide nanoparticles using banana extract as a capping agent, after drying it, it looks like thin sheets, cannot be grinded as powder (The last time, it does not look like...
12 June 2023 3,604 1 View
Safe cities for women with disabilities
13 May 2023 8,560 3 View
#DNA_extraction #QIAamp #Qiagen #Colony_DNA
04 May 2023 4,548 2 View
How to solve the problem if the sensor is not capable of transmitting data to base station in WSN. Is there any paper talking about this issue?
25 January 2023 5,399 4 View
i have precipitated enzyme by acetone.. when i use coomassie staining in native gel electrophoresis, it couldnot migrate to stacking and then resolving gel. is there is a problem in acetone...
10 January 2023 2,353 5 View
What is the most suitable experimental setting to understand the consequences of a particular gene: (1) targeted degradation of the specific transcription factor (TF) of the gene, followed by...
04 August 2024 6,265 1 View
I'm fairly new to making homemade comp cells and have done so with some mild success. What is generally an acceptable way of resuspending comp cells during wash steps? I've been told to swirl the...
31 July 2024 8,309 0 View
Genome-wide association study (GWAS) Continuous traits: eg. Height Discrete traits: eg. Eye color
28 July 2024 286 0 View
...
24 July 2024 3,087 3 View
I’m having difficulty achieving high RNA integrity in my samples. Although the 260/280 and 260/230 ratios are satisfactory after RNA extraction, the RNA samples show signs of degradation when...
22 July 2024 155 4 View
I want to study the differences between two groups: the treatment group and the comparison group. The total population consists of 60000 women in the treatment group, distributed across different...
21 July 2024 669 3 View
I want to extract glycosides from my mangrove leaf samples, but my laboratory lacks an incubator capable of reaching temperatures above 60°C. The paper I am following specifies a minimum...
13 July 2024 3,686 0 View
We're studying the stability and fusion behavior of multiple PVC compounds in a Brabender. After a few trials, we set the temperature at 195 °C and the rotor speed at 70 rpm. Samples differ in...
12 July 2024 2,768 1 View
Hello everyone, I am trying to encapsulate an essential oil in the liposomes using the heating method and encountering some issues. I am following the protocol described below, but my solution...
10 July 2024 4,083 3 View
Is any specific sample preparation required to perform VSM measurement of NdFeB alloy powder to get a square-shaped M-H loop? Or is it mandatory to make magnetically aligned and sintered pellets...
09 July 2024 1,969 0 View