I have frozen human tissue samples and I need to measure ADP/ATP ratio in these tissues using a bioluminescence kit from Abcam. what is the best method to prepare my samples without degrading ADP or ATP?
I use para nitrophenyl palmitate substrate with tris buffer ph 8... i read many temperature for Yellow color of para nitro phenol production.. 37 and 50 and 40 at water bath ... I confused as i...
04 February 2021 9,100 5 View
If I want to know the changes (for example in plants or soils) as affected by specific substance on nano substance; when I use SEM or TEM and why? As well, when I use FTIR or EDX and why also? Is...
28 January 2021 5,070 9 View
iam working on long memory time series data and i use lstm neural networ to train data and i want to understand how rmse is calculated is it calculated in each iteration separately or in each...
23 December 2020 6,740 4 View
if the percentage of doping Au into rGO is too small 1~10%, is it not enough to prevent restacking of r-GO in xrd results, rGO peak appeared at 22.2 and also the peaks of gold nanoparticles so...
21 December 2020 6,091 3 View
is the concentration of gold salt that were added will affect on the peaks appeared. i added a very little amount of gold salt to dope graphene oxide and not all the peaks that proved the...
17 December 2020 9,275 3 View
How to calculate the inductance and resistance of a 'multi-winding transformer MATLAB/Simulink block'?
07 December 2020 8,262 3 View
i am trying to dope boron in graphene oxide , and after i take out my samples from the oven i put them in the microwave for 40 sec , and i saw sparkles . and fume also i couldn't understand why
16 November 2020 6,852 5 View
i am looking for efficient texture analysis software for MRI images analysis with free access?
09 October 2020 3,737 2 View
I have Pre test as a (covariate variable) and post test as a (dependent variable) and the treatment for the experimental group and the control group. and the assumption of Homogeneity of...
21 August 2020 7,196 3 View
What you should do if you found that one of the reviewers goes through your paper just to reject it and most of his comments are irrational and not constructive. Finally, he rejected the paper...
20 April 2020 5,336 51 View
01 March 2021 3,905 5 View
I personally feel this as a most confusing question. As per my understanding, if sample size is > 30, and sample is skewed, we can still use paramteric approach, as it will follow Z...
01 March 2021 1,587 6 View
Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...
01 March 2021 8,169 2 View
Good day to all, I have no experience with light emitting diodes. I am building a glass mini flume to demonstrate the photo degradation of Methylene Blue in water running over self cleaning...
26 February 2021 4,026 5 View
Hi all The question is related to TGA(Thermogravimetric analysis). Thermal degradation easily understood but why do samples weight decrease? altough just binding is broked, the weight of...
26 February 2021 1,100 5 View
How do I run a a priori sample size determination for non-parametric tests, like the Mann-Whitney U-test and the Kruskall-Wallis or other non-parametric tests that accord with the unpaired t-test...
24 February 2021 166 6 View
I need to conduct a a priori sample size determinations for t-test with unequal means, but I don't know the sizes of the groups nor the ratio of both groups... Can I do a sample size determination...
24 February 2021 8,289 3 View
I'm a chemistry student and I'm researching the kinetic degradation of mixed plastic waste. I'm having trouble calculating the activation energy by the coats & Redfern method. The data that...
24 February 2021 974 1 View
My sample size is too small to conduct a factor analysis. I created my own psychometric scale for measuring Effectiveness of Support, which contains 38 items (4 sub-scales) Im really struggling...
23 February 2021 9,287 12 View
23 February 2021 8,850 2 View