A single subcutaneous injection of 25 mg DMBA mixed with 0.5 mL sunflower oil and 0.5 mL sodium chloride 0.9% would be enought for tumor formation in Sprague Dawley female rats, aged 60 days. Control group received 0.5 ml of sunflower oil and 0.5 ml of saline only. Tumor detection by palpation is successful after 8-10 weeks.
7,12-Dimethylbenz[a]anthracene (DMBA) is a synthetic carcinogen/chemical which belongs to the PAH family and efficiently induced ductal carcinomas through the process of mammary carcinogenesis in rats within eight to ten weeks.