Hello all
I want to induce chondrogenic differentiation in my adipose derived stem cells and dental pulp stem cells. As I'm going through literature, I have found varied recipes for chondrogenic differentiation induction medium, some using TGF beta 1 and others using TGF beta 3. I'm unable to comprehend which supplement is better for the induction. Can anyone please provide an explanation to this.
Hi,
I would recommend TGF- beta 3. If you also want to know more about the recipe for the chondrogenic differentiation medium and culture and expansion I would recommend referring Estes et al. 2010, Nature Protocols. The authors have explained the culture and expansion of human ADSCs and also the characterization.
Hope this helps. Good luck!
to be honest TGFB1 + 3 will induce differentiation when supplemented with ITS, aerobic acid, Dexamethasone.
why not compare both with your cells and then decide after comparison at multiple timepoints 7, 14, 21 days etc by running a panel of genes IE: PRG4, ACAN, SOX9, COL2, COL1, COLX, RUNX2, ALP, SP1.
good luck
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