I need to establish some alternative or additional techniques for analysing biomarkers of insulin resistance in blood plasma.
Thank you Rodolphe! I'll research those options!
Hi everyone, As part of my PhD project, I am conducting mixed ANOVA's in SPSS. I understand how to run the procedure and have been using the SPSS Survival Manual by Julie Pallant as my main...
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I understand that if you collect data pre and post-intervention from the same individuals, a paired sample t-test is the best option to compare differences between the scores. However, if a few...
06 July 2018 8,123 5 View
I have conducted a human intervention study and measured various physical and metabolic characteristics pre- and post-intervention. I would like to conduct stepwise backward regression in SPSS to...
31 December 2017 4,553 9 View
I have ran multiple mixed ANOVA's on results I obtained during a recent study. I understand how to conduct a mixed ANOVA but I'm having trouble with satisfying certain assumptions of this...
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Looking for recommendations of highly regarded academic/research conferences on physical inactivity, sedentary behaviour and health taking place next year in 2018.
10 November 2017 4,143 4 View
Looking for different methods to reduce the metabolic activity of cells. Primarily HepG2 cells, but muscle or adipose tissue cells either.
05 June 2017 1,668 4 View
I have two intervention groups with uneven subjects. When I run a two-way RM ANOVA it takes the means of the smaller group thus I presume the ANOVA results are incorrect. Is there a way of...
03 April 2017 6,152 10 View
I have been getting a low plasma and no coating while doing rf sputtering of copper doped ZnS using a power of 140 watts and at pressure of 6.5x10-3 Mb.
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i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
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Women, on the other hand, can become physically aroused (increased blood flow in the reproductive organs) without becoming psychologically aroused even in the slightest. (Robert Weiss)
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In my study, I intend to infect PBMCs with SARS-CoV-2. After that, I will analyze NK cells by flow cytometry to see if their phenotype changes or if they show degranulation. After the infection, I...
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I am trying to prepare samples for IP. The pellet size is good and I am using 1% Tx-100 and using a syringe to further lyse the cells and maintain protein-protein interactions. I keep having a...
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I want to perform a CRISPRa/i library screening for candidate regulatory factors that affect pathogenic Th17 differentiation in PBMC. But I am wondering how to define the size of gene library, so...
31 July 2024 2,150 0 View
I am developing an ELISA kit for rotavirus A (G1P8 and G1P6). Does anyone know the weight (ng) of these two strains? Or to help me find their weight?
29 July 2024 3,138 2 View
Please address the best way to drop a plasmid. Background: I have a "bait" plasmid resistant to kanamycin and a "prey" plasmid resistant to carbenicillin. After many rounds of streaking on...
25 July 2024 2,532 3 View
I am using the Polyomics Integrated Metabolomics Pipeline (PiMP) for metabolite feature analysis. After annotating my data, I have encountered cases where multiple potential metabolites are...
24 July 2024 5,789 0 View