What to do if Levene's test is significant in a mixed ANOVA in SPSS?

Hi everyone, As part of my PhD project, I am conducting mixed ANOVA's in SPSS. I understand how to run the procedure and have been using the SPSS Survival Manual by Julie Pallant as my main...

10 November 2018 815 8 View

A paired sample t-test for unequal sample sizes?

I understand that if you collect data pre and post-intervention from the same individuals, a paired sample t-test is the best option to compare differences between the scores. However, if a few...

06 July 2018 8,072 5 View

What are the correct values to use for stepwise backward regression from an intervention study?

I have conducted a human intervention study and measured various physical and metabolic characteristics pre- and post-intervention. I would like to conduct stepwise backward regression in SPSS to...

31 December 2017 4,493 9 View

Can anyone help me with assumptions relating to Mixed ANOVA's in SPSS please?

I have ran multiple mixed ANOVA's on results I obtained during a recent study. I understand how to conduct a mixed ANOVA but I'm having trouble with satisfying certain assumptions of this...

10 November 2017 4,222 4 View

Conferences relating to physical inactivity, sedentary behaviour and health taking place in 2018?

Looking for recommendations of highly regarded academic/research conferences on physical inactivity, sedentary behaviour and health taking place next year in 2018.

10 November 2017 4,081 4 View

Ways to reduce the metabolic activity of cells in-vitro?

Looking for different methods to reduce the metabolic activity of cells. Primarily HepG2 cells, but muscle or adipose tissue cells either.

05 June 2017 1,579 4 View

Does anyone know how to account for uneven sample sizes using a two-way repeated measures ANOVA in SPSS?

I have two intervention groups with uneven subjects. When I run a two-way RM ANOVA it takes the means of the smaller group thus I presume the ANOVA results are incorrect. Is there a way of...

03 April 2017 6,098 10 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View