Hi, how should one statistically handle technical duplicates/triplicates? Should you first find the mean and then treat it as a single measurement or should you handle each repeat as an individual sample? I have in mind things like qPCR, elisa...
Hello, Do any of you have experience with the analysis of chest wall compartments using a 32-marker model? Currently, we are using the standard 89-marker model, which is very time-consuming. Is...
26 October 2023 9,124 1 View
Hello. This year we started using ITC in a bit unconventional way to study enzyme kinetics of beta-lactamase (there is already literature data that showed it works). The principle is fairly...
06 September 2023 1,801 3 View
Hello! I'm using Endnote online and need help finding a reference style similar to JSCR, as the JSCR official reference style is not in Endnote online. Can anyone help? Thank you!
24 July 2023 6,200 1 View
Each life form has its own viruses. I am wondering if Plasmodium has specific viruses as well and which could be utilized as a measure to reduce Malaria infections? A virus which infects a...
16 January 2023 4,922 2 View
Of course, phages do only interact with bacteria which have specific binding proteins. But why do only bacteria have these binding proteins? What if vertebrate cells would have these binding...
07 January 2023 1,583 4 View
Here is what I'm trying to do: to solvate a peptide in water and ions that would mimic a PBS solution. To do this I tried uploading my peptide into the Solution Builder, adding the following...
13 December 2022 4,331 0 View
Dear RG community, I'm starting a photoacoustic project, and I need to acquire some ultrasonic receivers. Acoustics is not my expertise, so I'm asking here for help! :D Where to buy, what is...
28 September 2022 4,408 6 View
Hi there, I am a student who is very new to ANSYS. I want to simulate the following scenario A flexible rod is connected to a rigid hub. The hub starts from 0 rad/s at 0ͦangle and reaches 100...
25 September 2022 1,560 0 View
The photos are from the inland of Sao Miguel - Azores.
22 September 2022 1,972 5 View
With ongoing development of real-time "Free Style Libre" insulin detectors, has anyone ever did insulin analysis over a week period for a wild "forest" mice (not wild type lab mice)?
15 August 2022 7,487 4 View
I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.
11 August 2024 9,101 4 View
I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...
10 August 2024 7,429 2 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
Hello experts, Does anyone know any free software about retention index prediction ?
08 August 2024 7,403 2 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
I am trying to analyse data from a survey examining what variables affect teachers perceived barriers to incorporating technology into their classroom. I have 5 predictor variables however my DV...
06 August 2024 1,752 3 View
To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...
06 August 2024 6,728 6 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
Women, on the other hand, can become physically aroused (increased blood flow in the reproductive organs) without becoming psychologically aroused even in the slightest. (Robert Weiss)
05 August 2024 9,537 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View