What is the maximum number of reads per one run with NGS?

Does anybody know how many reads are usually generated with Illumina NextSeq? For sequencing plasmid library (100-150 bp per read), for example.

27 September 2020 799 2 View

Hello dear researchers, how do you remove the black foil from the vine nursery?

Hello, how do you remove the black foil from the vine nursery? Attached picture in the file. is there machine way or by hand?

04 September 2020 3,566 2 View

Glioblastoma GL-261 cell line not growing s.c?

Hi has anyone had experiance of GL-261 not growing subcutaneously? I have inoculated 10e6 cells in C57BL/6J female mice and the tumors became palpable after a week cca but have subsequently...

19 February 2020 7,543 2 View

Microplastic extraction from fish guts using soluble filters?

Hi there, I want to isolate microplastic particles from fish guts and water samples. My initial idea was to extract guts from selected specimens and then dissolve the whole guts using 10% KOH...

12 February 2020 4,467 8 View

Why are my glucose levels decreasing very rapidly?

Hello, I'm conducting an IPGTT experiment on WT and KO mice (mice which we have shown to have improved glucose tolerance) and I have a serious issue of glucose levels plummeting after 30-60min....

28 September 2018 962 4 View

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10 December 2015 1,475 1 View

How can I calculate deflection of steel fiber reinforced concrete beams?

How can I calculate deflection of SFRC beams (ordinary and high strength)? Is there ACI or other proposals?

11 May 2015 4,977 14 View

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I need approximately 100 meters of ceramic cord (0,5mm). I need it for attaching extensometer to the tensile specimen when conducting tensile test at elevated temperatures.

10 November 2014 4,572 3 View

What is the best bacterial vector for large inserts (around 50 kb)?

Is it possible to insert 50 Kb of DNA to a bacterial vector and successfully maintain and amplify it in bacteria? If so, what would be the best vector?

12 January 2014 8,832 2 View

Where could I find complete interviews of some convicted Western jihadists?

I am NOT looking for studies on the subject, but actual interviews.

01 January 1970 5,977 2 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

Why are the velocities so high in my Boussinesq approximation Fluent simulation?

Hello, I have a mechanism where air rises due to convective flows. In this simulation, nothing is driving the air except this. I understand that the Boussinesq approximation only works for small...

02 March 2021 4,910 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Which is a lucid standard comprehensive book to guide on psychological scale construction and its standardization?

02 March 2021 7,082 3 View