How can I measure Superoxide dismutase (SOD)?

Hi everyone, SOD measurement need to making assay buffer (Na-PO4 buffer+ NBT, L.Methionine and Riboflavin). I prepare this buffer under dark condition but unfortunately after about 10 -15 min its...

08 September 2018 8,694 6 View

Does anyone have a protocol for preparation of Fe-EDDHA for using in Hoagland nutrient solution?

08 September 2016 9,355 0 View

How can I measure nicotianamine (NA) concentration?

After going through some papers, I found that the liquid chromatography-mass spectrometric (LC-MS) is a good way for analysis of NA, but I have no access to LC-MS. Would you please suggest me...

07 August 2016 8,837 5 View

What are the exact differences between Fe2O3 nanoparticles and nano-iron chelat?

Hi,Would you please tell me what the exact differences between Fe2O3 nanoparticles and nano-iron chelat are?Thanks

07 August 2016 6,708 0 View

Iron

Calcareous soil is a dominant kind of soil in our country that causes a lot of problems for plant production. So, I decide to work on correction of iron deficiency in the physiology field as my...

05 June 2016 7,307 1 View

Does anyone have a good suggestion about iron nutrition of ornamental plants?

Calcareous soil is a dominant kind of soil in our country that causes a lot of problems for plant production. So, I decide to work on correction of iron deficiency of ornamental plants in the...

05 June 2016 703 4 View

Chlorophyll molecule synthesis

Hi, How much time will take a Chlorophyll molecule synthesis? Is it known? Thanks

01 January 1970 546 4 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Does the pressure(-600 mmHg) affects pH?

When I conducted the degassing experiment using decompression under anaerobic condition(and I used ferrous based chelating agent), the oxidized ferric were observerd. So, could you tell me the...

03 March 2021 4,892 5 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

No title

02 March 2021 3,060 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Which bacteria are suitable for quorum sensing screening?

I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...

01 March 2021 7,675 3 View