Hi everyone,
SOD measurement need to making assay buffer (Na-PO4 buffer+ NBT, L.Methionine and Riboflavin). I prepare this buffer under dark condition but unfortunately after about 10 -15 min its color change to blue. I don't know why?
Do someone know the reason of this problem?
Thanks
Hello @ Zeinab I did measure SOD some couple of years back. Please, find the following links and see if they would be able to help.
Article The Effects of Rooibos (Aspalathus linearis), Green Tea (Cam...
Article Protective effects of rooibos (Aspalathus linearis), green t...
SOD estimation by Bichop and Freidrich method.
The assay system consisted, 200μl of 50 mM
sodium carbonate, 400μl of 0.1 mM EDTA, 800μl of 25μM
NBT. 800μl of 1mM hydroxylamine hydrochloride and
100μl of sample in a cuvette. Then incubate for 15 minutes
under tungsten lamp. Measure absorbance at 560 nm. Then
specific activity of each sample was estimated in U/min/mg
of protein, by using the formula:
AA/min/vol of sample/mg of protein/extinction co-efficients x dilution factor
3 Here; Vol of sample =0.l ml;
@ Proteek Dasgupta Thanks Proteek, would you please tell me about AA AND extinction co-efficients ?
AA/min/vol of sample/mg of protein/extinction co-efficients x dilution factor Here; Vol of sample =0.l ml; extinction co-officient = 7.4 x 103 ;dilution factor
=23 ml (volume of assay).
AA = CHANGE IN ABSORBANCE
Hey Zeinab Izadi Apologies for the delay in response. Please revert back if there are any issues with the reply.
Cheers:-)
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