How can I measure Superoxide dismutase (SOD)?

Hi everyone, SOD measurement need to making assay buffer (Na-PO4 buffer+ NBT, L.Methionine and Riboflavin). I prepare this buffer under dark condition but unfortunately after about 10 -15 min its...

08 September 2018 8,694 6 View

TDZ absorption through cell membran?

Hi TDZ always dissolve in small volume of NaOH. TDZ PKa is 8.86. Therefor in high pH it is not ionic. Can be TDZ absorbed through cell membrane in non-ionic form ? Thanks

10 November 2017 1,685 0 View

Does anyone have a protocol for preparation of Fe-EDDHA for using in Hoagland nutrient solution?

08 September 2016 9,355 0 View

How can I measure nicotianamine (NA) concentration?

After going through some papers, I found that the liquid chromatography-mass spectrometric (LC-MS) is a good way for analysis of NA, but I have no access to LC-MS. Would you please suggest me...

07 August 2016 8,837 5 View

What are the exact differences between Fe2O3 nanoparticles and nano-iron chelat?

Hi,Would you please tell me what the exact differences between Fe2O3 nanoparticles and nano-iron chelat are?Thanks

07 August 2016 6,708 0 View

Iron

Calcareous soil is a dominant kind of soil in our country that causes a lot of problems for plant production. So, I decide to work on correction of iron deficiency in the physiology field as my...

05 June 2016 7,307 1 View

Does anyone have a good suggestion about iron nutrition of ornamental plants?

Calcareous soil is a dominant kind of soil in our country that causes a lot of problems for plant production. So, I decide to work on correction of iron deficiency of ornamental plants in the...

05 June 2016 703 4 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View

Any Homemade Organic Synthesis Practices?

Hi everyone, I am a teacher of Heterocycles Synthesis, Is there a home practice that you recommend for undergraduate students? A simulator would also be useful Due to the pandemic, students...

18 February 2021 2,769 4 View

How to predict the reaction pathway for electro organic synthesis?

Can anyone recommend some any software/models available that can predict substance reactivities and the reaction yield under a specific set of reaction conditions process and proposing pathways to...

17 February 2021 2,383 3 View

Has anyone encountered any problems with abm good microRNA primers for quantification of mature miRNAs?

I've been trying to quantitate mature microRNAs in 293T cells with and without 'X'. 2ug of total RNA (isolated by Trizol method) is converted to cDNA using All-in one microRNA cDNA synthesis kit...

17 February 2021 8,986 2 View

How to measure acetic anhydride solution with 18M sulfuric acid?

I am doing research on the synthesis of paracetamol. I am preparing acetic anhydride solution with a few drops a sulfuric acid and wish to measure the pH of this solution. However my pH meter...

14 February 2021 8,699 3 View

Best synthesis method for large scale silver nanoparticle synthesis with high yield?.Best post processing steps after nanoparticle synthesis?

I have chemically synthesized silver nanoparticle to combat microbes on wound site. I have used many methods of synthesis, but I found minimal field of silver NP product.I am getting whitish...

11 February 2021 4,964 13 View

CDNA synthesis or qPCR did not work?

Hi everyone! I extracted RNA from urine pellet using phenol-chloroform method. The yield of RNA was 160ng/uL. 3uL of RNA was used for cDNA synthesis in 20 uL reaction using Go-Script kit....

05 February 2021 6,587 4 View

Polymer synthesis simulation - which software?

Hello, I am a synthetic polymer chemist. I would like to explore the simulation counterpart of my experimental work. Can anyone suggest a good software able to simulate polymerization reactions,...

04 February 2021 2,672 4 View