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Questions related from Olumuyiwa Igbalajobi
I have been trying to join 3 fragments (3kb, 2kb and 1kb respectively) in the last days using the DJ PCR protocol but to no avail. My 2nd PCR annealing temperature was at 57°degree for 10 min and...
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Is there any specific sequence different from TATA for the TATA box in fungi?i had about 61 base pairs deletion upstream and 462 base pairs deletion downstream of the first transcription ATG but...
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For months now i have been trying to transform a recipient strain of Aspergillus fumigatus with my gene of interest.Kindly help with an efficient protocol please. I often used 2ug of DNA as my...
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I did double digestion overnight,purify and used T4 DNA ligase,incubate overnight again and proceed to transformation the following day having used ratio 1:3 Vector:insert.However few colonies...
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