6 Questions 13 Answers 0 Followers
Questions related from Olumuyiwa Igbalajobi
I have been trying to join 3 fragments (3kb, 2kb and 1kb respectively) in the last days using the DJ PCR protocol but to no avail. My 2nd PCR annealing temperature was at 57°degree for 10 min and...
31 July 2020 1,765 2 View
Is it possible for the next ATG in frame after the deletion of the ORF ATG still translates to a functional protein?
14 June 2017 4,392 6 View
Is there any specific sequence different from TATA for the TATA box in fungi?i had about 61 base pairs deletion upstream and 462 base pairs deletion downstream of the first transcription ATG but...
23 May 2017 10,195 3 View
Am interested in getting mutant using the CRISPR cas 9 gene editing technology. However, having taken into consideration of no g after the PAM,still no true mutant. Any idea of a better method?Thanks
26 April 2017 3,316 4 View
For months now i have been trying to transform a recipient strain of Aspergillus fumigatus with my gene of interest.Kindly help with an efficient protocol please. I often used 2ug of DNA as my...
17 December 2015 2,979 3 View
I did double digestion overnight,purify and used T4 DNA ligase,incubate overnight again and proceed to transformation the following day having used ratio 1:3 Vector:insert.However few colonies...
05 December 2015 9,185 5 View
