For months now i have been trying to transform a recipient strain of Aspergillus fumigatus with my gene of interest.Kindly help with an efficient protocol please. I often used 2ug of DNA as my insert but to no success.
Hello Olumuyiwa
What protocols and transformation techniques have you been using previously?
Fresh conidia at 240rpm for 18hrs, 37 degrees , digest with novozyme to get protoplast and later used PEG solution among other procedures
Since you're using PEG, have a look at our publication (attached) for comparison. You will need to optimise any protocol for your specific setup anyway. Did you do any positive controls? Start by checking whether your protoplasts are still viable by plating them on medium without antibiotic.
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