Hi I need to develop an aurora panel of exhaustion markers of T cells. PBMC or patients who had chemo before. I did the literature and there are soooo many markers, I will look for surface markers

CD25, CD69, CD57, CTLA-4, LAG-3, PD1, TIM3, TIGIT

and intracellular

Foxp3, Eomes, Tbet, Helios, TOX

These ones seem absolutely necessary.

Please mark any surface or intracellular markers to absolutely not miss (2B4, CD39?).

Your help is greatly appreciated.

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