Gayane Garland

6 Questions 3 Answers 0 Followers

Questions related from Gayane Garland

Activation of CAR-T cells with CD19 coated beads for intracellular staining?

Hi, I have incubated my anti-CD19 CAR-T cells with CD19 coated beads (upped to 5 beads for 1 cell in my last experiment, transduction rate 30%), 3 hours later I have added protein export inhibitor...

01 March 2025 8,654 0 View

HepG2 cell proliferation is boosted by T cells ?

Hello, We are culturing HepG2 cells in low attachment wells to make them grow in shpheroid forms in RPMI 10% SVF. Then T cells are added to the wells and the culture medium becomes 50% RPMI 10%...

22 January 2025 7,936 0 View

Gata-3, Eomes, Ror gamma T intracellular transcription factors?

Hi, does anyone have a good protocol for Gata-3 staining in particular. I have 0 staining, with my protocol (BD FoxP3 staining buffer) while Tbet worked well for example? thank you in advance

06 January 2023 3,728 1 View

BV, BUV versus cFluor fluorochromes from cytek?

Hi, has anyone worked with these fluorochromes? Cytek cFluor antibodies are very expensive, but I am wondering whether they are better for 30+ marker panels in terms of compensation errors. Thank...

01 December 2022 1,396 0 View

Flowjo vs Omiq vs Cytobank which one to choose for a panel with 20+ markers, flow cytometry?

Hi, i have now a fully working panel with lots of markers and will proceed to stain and compare samples, pmbc healthy vs non using flow cytometry. Which one of these programs to choose for...

23 November 2022 5,952 4 View

T cell exhaustion markers for an aurora panel?

Hi I need to develop an aurora panel of exhaustion markers of T cells. PBMC or patients who had chemo before. I did the literature and there are soooo many markers, I will look for surface markers...

08 November 2022 4,084 0 View