I have entered data into SPSS but need to know how to make zero equal a non responses in SPSS, thank you for your help?

I have entered data into SPSS but need to know how to make zero equal a non responses in SPSS, thank you for your help?

23 February 2016 2,066 6 View

I have entered data into SPSS but need to know how to make zero equal a non responses in SPSS, thank you for your help?

I have entered data into SPSS but need to know how to make zero equal a non responses in SPSS, thank you for your help?

01 February 2016 9,632 7 View

I have used SPSS to analyze my data and the KMO value is .848 which is positive, I am would like to ask what does this mean for my survey tool?

This tool was created by me as a way to measure students thoughts on cultual capital (embodied) for this one specifically. The questionnaire is 25 questions on a likert scale of strongly disagree,...

08 September 2015 5,711 14 View

What type of statistical analyses should I use?

i have data from 500 participants who responded to 61 questions using a lickert scale (SA-SWA-A-Maybe-DA-SWD-SD) I want to know the rate of agreement/disagreement with the 61 questions, which...

01 February 2015 8,402 20 View

How long can I keep the master mix cocktail of conjugated antibodies?

Hi everyone, I made a mistake when I computed how much antibody I needed for my FACs, so I have an excess of exactly 10 reactions. Since they are so expensive and our lab is so poor, I was...

27 October 2020 3,531 3 View

Could you recommend a PhosFlow-compatible human CD27 antibody?

I am measuring phosphorylated STAT5 in different lymphocyte populations using flow cytometry in response to stimulus. I need to accurately gate different memory populations using CD27 (not CCR7,...

30 July 2020 3,378 2 View

Should FMO controls be prepared for all samples to be used in the experiment or is it sufficient to prepare for a single sample?

In the flow cytometry experiment I will perform soon, I will use the unstained control to detect autofluorescence, the single stain control as a compensation control to determine the degree of...

22 June 2020 5,269 4 View

The compensation procedure- beads (for fluorochrome) and cells (dead/live staining)- how to do it?

Hi, I would like to ask about the compensation procedure. I use Fortessa/ Diva and automated compensation procedure. I have a set from BD for compensation setting. Referring to the manufacturer...

28 February 2020 8,765 2 View

Is there a protocol for a multicolour fluorochrome cell staining in microplates?

Our laboratory is not equipped with a flow cytometer. Is it possible to stain cell suspensions (i.e. from thymus) in 96 well plates with multiple fluorochrome conjugated antibodies and then read...

23 February 2020 1,566 3 View

Can I use an antibody with application for FC, to do IP or WB??

Hi there! I need to do immunoprecipitation and Western blot to obtain and detect a protein and I already have conjugated antibodies with FITC and PE for flow cytometry. I was wondering if there...

17 February 2020 223 3 View

Primary antibodies conjugated with fluorochrome is suitable for IHC?

01 February 2020 3,003 3 View

Can I use an antibody with application for FC, to do IP or WB??

Hi there! I need to do immunoprecipitation and Western blot to obtain and detect a protein and I already have conjugated antibodies with FITC and PE for flow cytometry. I was wondering if there...

01 February 2020 2,440 3 View

Is there a protocol for a multicolour fluorochrome cell staining in microplates?

Our laboratory is not equipped with a flow cytometer. Is it possible to stain cell suspensions (i.e. from thymus) in 96 well plates with multiple fluorochrome conjugated antibodies and then read...

01 February 2020 7,997 2 View

Flow Cytometry: CD3 Market in Jurkat T Cells?

Hello! I have been working with Jurkat T Cells for flow cytometry. I noticed that although Jurkat cells are T cells, their positive signals for CD3 antibodies were not so high. I've used 4-5 CD3...

09 October 2019 7,320 7 View