Hi, has anyone worked with these fluorochromes? Cytek cFluor antibodies are very expensive, but I am wondering whether they are better for 30+ marker panels in terms of compensation errors.
Thank you for your input
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Hi I need to develop an aurora panel of exhaustion markers of T cells. PBMC or patients who had chemo before. I did the literature and there are soooo many markers, I will look for surface markers...
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Others have recommended that I dry soil using an oven, and then re-apply water to attain the required level of moisture (10%). One recommendation was to use 1mL water per 10g dry soil to reach 10%...
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I have entered data into SPSS but need to know how to make zero equal a non responses in SPSS, thank you for your help?
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I would like to use FAM [5(6)-carboxyfluorescein] (ex 493/emission 517) fluorochrome in a Immunofluorescence protocol and I need to fix the staining. I can't find any fixation protocol convenient...
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I am currently working on designing a flow cytometry protocol to detect apoptosis in honeybee Kenyon Cells. However, I am not sure which fluorochromes or antibodies I can use to identify...
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I would like to do an IF of alpha1-syntrophin on PE brain tissue but I detect no signal. I already tried 2 different ways to proceed antigen retrieval (AR): heat in citrate buffer at 95°C or...
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Dear all, I realized that Biolegend has all its Treg staining kits as AF488 conjugated FoxP3 and other dyes for CD25/CD4. What is the reason behind it that they are changing CD25 and CD4...
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My protein having his tag which I have purified using Ni-NTA chromatography. I want to use purified protein for Cell culture studies on Macrophages. To study entry of protein into the cell, I want...
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I am carrying out Western blot to determine effects of extracts on expression of LPS induced iNOS in murine macrophage cells, I am using the iBind flex system from Thermofisher for multiplexing...
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Various fluorochromes such as CMA3 and DAPI are used in chromosome banding. Base-specific fluorochromes such as CMA3 (GC) and DAPI (AT) are also used to distinguish heterochromatic segments that...
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Hi everyone, I made a mistake when I computed how much antibody I needed for my FACs, so I have an excess of exactly 10 reactions. Since they are so expensive and our lab is so poor, I was...
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