Dear colleagues,
Could anyone explain to me How I can synchronize 3T3-L1 preadipocyte cell lines that will be cultured in DMEM–Dulbecco's Modified Eagle Medium.
Your cooperation is highly appreciated.
Dear friends I am optimizing WB on liver tissue collected from mice and I need an answer for above mentioned question according to your expertise?
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hello everybody, I would like to measure Hepatic insulin sensitivity both in vivo and cultured cells. Thus, I am looking for new methods other than traditional previous approaches such as (glucose...
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Could anyone recommend the best extraction kit for me in order to extract RNA from bacterial culture? Your cooperation is highly appereciated. Thanks in advance.
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Anybody can help me to find an equation to calculate effect size of SNPs?
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If we manipulate gene expression in adipocyte culture to affect insulin sensitivity. Which are the suitable methods for measuring insulin sensitivity??
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Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
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Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
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I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
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Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
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I am currently looking for a questionnaire that relates with my study which is The Effectiveness of Organizational Culture towards employee retention rates
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I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...
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