Could I use 2ndry Ab (Goat Anti-Rabbit Immunoglobulins/HRP) several times in detection stage of WB once it is diluted in 5% BSA or milk/TTBS ?

Dear friends I am optimizing WB on liver tissue collected from mice and I need an answer for above mentioned question according to your expertise?

07 September 2020 1,607 3 View

New methods for measurement hepatic insulin sensitivity both in vivo and cultured cells?

hello everybody, I would like to measure Hepatic insulin sensitivity both in vivo and cultured cells. Thus, I am looking for new methods other than traditional previous approaches such as (glucose...

21 December 2019 6,557 1 View

New methods for measurement hepatic insulin sensitivity both in vivo and cultured cells?

hello everybody, I would like to measure Hepatic insulin sensitivity both in vivo and cultured cells. Thus, I am looking for new methods other than traditional previous approaches such as (glucose...

11 December 2019 4,911 1 View

What is the best way for extraction RNA from bacterial culture?

Could anyone recommend the best extraction kit for me in order to extract RNA from bacterial culture? Your cooperation is highly appereciated. Thanks in advance.

02 March 2019 3,508 3 View

How can we measure insulin sensitivity in adipocyte culture?

If we manipulate gene expression in adipocyte culture to affect insulin sensitivity. Which are the suitable methods for measuring insulin sensitivity??

08 September 2018 1,056 0 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View