If we manipulate gene expression in adipocyte culture to affect insulin sensitivity. Which are the suitable methods for measuring insulin sensitivity??
Dear friends I am optimizing WB on liver tissue collected from mice and I need an answer for above mentioned question according to your expertise?
07 September 2020 1,775 3 View
hello everybody, I would like to measure Hepatic insulin sensitivity both in vivo and cultured cells. Thus, I am looking for new methods other than traditional previous approaches such as (glucose...
21 December 2019 6,747 1 View
11 December 2019 5,099 1 View
Could anyone recommend the best extraction kit for me in order to extract RNA from bacterial culture? Your cooperation is highly appereciated. Thanks in advance.
07 March 2019 3,069 3 View
02 March 2019 3,649 3 View
Dear colleagues, Could anyone explain to me How I can synchronize 3T3-L1 preadipocyte cell lines that will be cultured in DMEM–Dulbecco's Modified Eagle Medium. Your cooperation is highly...
11 December 2018 6,780 0 View
Anybody can help me to find an equation to calculate effect size of SNPs?
14 September 2018 697 4 View
I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...
05 August 2024 6,787 4 View
I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....
05 August 2024 2,570 3 View
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05 August 2024 4,407 0 View
I am trying to prepare samples for IP. The pellet size is good and I am using 1% Tx-100 and using a syringe to further lyse the cells and maintain protein-protein interactions. I keep having a...
01 August 2024 7,199 1 View
I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...
31 July 2024 225 2 View
I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...
30 July 2024 6,420 1 View
I cannot confirm cell surface protein expression by flow cytometry even after transfection and antibiotic selection of cells. Does it take long for proteins to express on the cell surface? the...
28 July 2024 3,178 3 View
I created two potential gene expression cassettes (constitutive and inducible) for expression of a mutant PETase gene on PeptiCloud using the version tree feature, which allows users to create...
28 July 2024 7,559 1 View
How much volume of siRNA can I add to 2.5 mL of liposomes with a total lipid concentration of 10 mg/mL?
27 July 2024 6,920 1 View
Please address the best way to drop a plasmid. Background: I have a "bait" plasmid resistant to kanamycin and a "prey" plasmid resistant to carbenicillin. After many rounds of streaking on...
25 July 2024 2,532 3 View