Susbstitute for proteinase K in lysis buffer ?

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Avoiding clumping of DNA ladder?

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Preserving clones in glycerol stock?

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Katie A Burnette

In short, no. You can look for a different protocol.

Kamesh Krishnamoorthy

Thanks mam

Jai Ghosh

Actaually there is no substitute for proteinase k in the classical estimation method of DNA. However, one may find several sustitutes being worked upo like, a plant proteinase, extracted from Bromelia fastuosa, as an alternative to proteinase K