7 Questions 20 Answers 0 Followers
Questions related from Piotr Majewski
Dear All, could some please suggest proper PhiX concentration for multiplex Illumina sequencing with use of MiSeq kit v2 and Nextera XT. samples #1-11 are highly similar - around 50-56%GC sample...
08 September 2019 3,048 1 View
hi all, anyone has some advices considering bacterial genome assembly with use of Unicycler (@Galaxy)? I am trying to assemble paired-end data obtained from Illumina platform. Data was...
09 November 2017 3,869 4 View
I have attempted to linearize plasmids with EcoRI and XbaI. Below I upload a electrophoretic separation of 5 plasmid preps (uncut and RE digested). Can it be assumed (for example) that...
07 April 2017 676 19 View
Dear all, I need to dissolve templates for further analysis, namely for Sanger sequencing. It is acceptable to dissolve templates in DEPC-treated water? PS should I use Tris buffer (10mM Tris HCL,...
04 December 2015 5,076 3 View
Dear colleagues, could anyone give me some advices about performing proper reverse transcription of bacterial mRNA – which is really low-abundant in total isolated RNA (probably less than...
28 October 2015 1,273 14 View
I wonder if there is a possibility to perform purity and quantity measurement of PCR product after ExoSap treatment? What about blank sample in this case?
11 June 2015 9,228 6 View
Dear All, does anyone have a Enterobacter cloacae ST335 strain in their collection? I need such strain as a comparator in molecular analyses, therefore I am interested in establishing possibile...
01 January 1970 5,241 1 View
