Piotr Majewski

5 Questions 15 Answers 0 Followers

Questions related from Piotr Majewski

Suitable PhiX concentration for diversified libraries (MiSeq v2, Nextera XT)?

Dear All, could some please suggest proper PhiX concentration for multiplex Illumina sequencing with use of MiSeq kit v2 and Nextera XT. samples #1-11 are highly similar - around 50-56%GC sample...

08 September 2019 2,936 1 View

Unsuccessful bacterial genome assembly with use of Unicycler?

hi all, anyone has some advices considering bacterial genome assembly with use of Unicycler (@Galaxy)? I am trying to assemble paired-end data obtained from Illumina platform. Data was...

09 November 2017 3,815 4 View

Gel electrophoresis of unknown resistance plasmids?

I have attempted to linearize plasmids with EcoRI and XbaI. Below I upload a electrophoretic separation of 5 plasmid preps (uncut and RE digested). Can it be assumed (for example) that...

07 April 2017 607 19 View

Will DEPC-treated water negatively affect Sanger sequencing?

Dear all, I need to dissolve templates for further analysis, namely for Sanger sequencing. It is acceptable to dissolve templates in DEPC-treated water? PS should I use Tris buffer (10mM Tris HCL,...

04 December 2015 5,014 3 View

Any suggestions for an effective prokaryotic mRNA reverse transcription?

Dear colleagues, could anyone give me some advices about performing proper reverse transcription of bacterial mRNA – which is really low-abundant in total isolated RNA (probably less than...

28 October 2015 1,183 14 View