Hi!

You can use L929. It is so easy ptrotocol. Seed them 50,000 per cm2 and use for any antioxydant toxicity test. In parallel You can measure this antioxydant content in cell medium by spectrophotometry.

Reduced glutathione was the most important specific indicator in serum and extracted tissue cells , total antioxidant capacity also may use , so the oxidative stress must be detected by another parameters like total oxidant capacity and MDA that it more specific parameters 

You can monitor oxidative stress generated in the cells using CellROX and MitoSOX stain. CellROX can be used to monitor nuclear and cytosolic oxidative stress (or ROS) while MitoSOX (superoxide indicator) is specific for mitochondrial oxidative stress. You need to perform fluorescent imaging experiments after the treatment. We used SH-SY5Y, MCF-7, BV-2 and RAW cells.

Protocol for CellROX stain is given below;

Procedure:The human neuroblastoma cell line SH-SY5Y, was grown in DMEM-F-12 (1:1) medium, supplemented with 10% fetal bovine serum (FBS), 1% penicillin/streptomycin, and maintained at 37°C, 5% CO2 in a humidified incubator. Once cell reach 80 % confluence further experiment was performed. Solutions of compounds were prepared in DMEM-F12 media (without phenol red). These solutions were added to the cells and cells were then incubated for 18 hours. After incubation media was removed, CellROX green was added to the wells and incubated for 10 minutes. Then cells were washed with PBS. Cells were fixed using 4 % paraformaldehyde for 10 minutes. Then cells were washed Coverslips were removed from the plates and inverted onto the grease free slides. Slides were sealed with nail polish. Confocal live cell imaging for CellROX was performed using green filters (ex/em= 488/500-550 nm). 

In addition to this. you can also measure NO production in cells (like BV-2 and RAW) spectrophotometrically using Griess reagent. You can find this assays in supporting information of this article (http://onlinelibrary.wiley.com/doi/10.1002/slct.201600808/abstract)