How to optic buffer conditions for Protein interaction by SEC?

Hello all, I am relatively new to the field of Protein purification and FPLC. So, forgive my naive questions. I want to study if protein A and protein B interacts by SEC. However, the protein A...

31 December 2019 7,410 3 View

Good S-Phase marker for western blot or ICC?

04 May 2017 7,449 3 View

Exact opposite result in cell cycle phase at different time point after overexpression?

Hello, I did a general comparison of cell-cycle analysis between my protein/CNT overexpressed HEK cell poupation. However, after 24hour of transfection, it shows a 10% increase in S-phase whereas...

01 February 2017 869 6 View

Best cell synchronization method after transfection?

Hi, I am working with a slow proliferative cell line (doubling time 18-20 hours) and I want to perform cell cycle analysis by FACS after transient transfection with my protein of interest. I did...

01 February 2017 6,004 3 View

Is HEK293 a good model for studying effects on cell-cycle analysis?

31 December 2016 9,182 4 View

What is the best/quick way to check ADP-ribosylation?

09 October 2016 369 4 View

CoIP and strange results?

Hello all, I am looking for interacting candidates for my protein and after doing IP followed by mass spec (found twice), I found protein X to be enriched almost 1:1 ratio to my bait protein....

07 August 2016 3,168 7 View

Troubleshooting a IP-Mass spec strategy ?

Hello all, I am working with a protein domain which hypothetically can go into nucleus (as it contains two NLS). I wanted to do IP-Mass spec to see what kind of proteins it is interacting with....

06 July 2016 9,241 4 View

What is ideal time point for MG132 treatment?

Hi, I am planning to treat my cells (transiently transfected and I generally process transfected cells after 48 hours of transfection) with MG132 for preventing degradation. From literature, 10 um...

01 February 2016 3,478 7 View

What is the best buffer for maintaining transient nuclear protein interaction?

Hello, I am trying to find interacting protein partners for a small nuclear intracellular domain (which is my protein of interest). My plan is to do IP with nuclear extract followed by Mass...

31 December 2015 5,134 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

How does one access the Cranet survey data on HRM practices?

Hello, The Cranet website does not provide any lead to the data obtained from their multinational survey on HRM practices. Any help on accessing the data would be most appreciated. Thank...

01 March 2021 9,536 3 View

Is there any research regarding how training and development is going to change in the era of Fourth Industrial Revolution?

As the organizations getting prepared for the fourth industrial revolution, to enhance the skills of their workforce what type of changes are necessary and going to occur in the training and...

01 March 2021 3,199 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

How long do vaccines protect us against COVID 19?

Or will we have to get vaccinated every year, as in the case of influenza?

27 February 2021 1,048 5 View