13 Questions 28 Answers 0 Followers
Questions related from Tanmoyita Nayak
Hello all, I am relatively new to the field of Protein purification and FPLC. So, forgive my naive questions. I want to study if protein A and protein B interacts by SEC. However, the protein A...
01 January 2020 7,511 3 View
Hi, Could anybody suggest a good S-phase marker for WB or imaging? I am aware of PCNA and BrdU, but apart than that is there any protein highly expressed/ restricted to S-phase? Thanks.
05 May 2017 7,548 3 View
Hi, I am working with a slow proliferative cell line (doubling time 18-20 hours) and I want to perform cell cycle analysis by FACS after transient transfection with my protein of interest. I did...
02 February 2017 6,105 3 View
Hello, I did a general comparison of cell-cycle analysis between my protein/CNT overexpressed HEK cell poupation. However, after 24hour of transfection, it shows a 10% increase in S-phase whereas...
02 February 2017 960 6 View
Hello all, I want to study the effect of protein X on cell-cycle progression based on a AP-MS data in HEK cells where several cyclin and MCM proteins were upregulated. But digging into deeper on...
01 January 2017 9,279 4 View
Hello, Can anyone, who is familiar with ADP-ribosylation, suggest me what is the best way to check if my protein is ADP-ribosylated? Thanks.
10 October 2016 519 4 View
Hi, I want to check whether my protein X has any effect on global translation rate. I will use HEK cells for this experiment. One option is polysome profiling, but any other assay? Thanks.
08 August 2016 8,708 2 View
Hello all, I am looking for interacting candidates for my protein and after doing IP followed by mass spec (found twice), I found protein X to be enriched almost 1:1 ratio to my bait protein....
08 August 2016 3,287 7 View
Hello all, I am working with a protein domain which hypothetically can go into nucleus (as it contains two NLS). I wanted to do IP-Mass spec to see what kind of proteins it is interacting with....
07 July 2016 9,351 4 View
Hi, I am planning to treat my cells (transiently transfected and I generally process transfected cells after 48 hours of transfection) with MG132 for preventing degradation. From literature, 10 um...
02 February 2016 3,628 7 View
Hello, I am trying to find interacting protein partners for a small nuclear intracellular domain (which is my protein of interest). My plan is to do IP with nuclear extract followed by Mass...
01 January 2016 5,249 3 View
Running a 10-20% Tris-Tricine blot with nuclear and cytoplasmic fraction of cell lines transfected with 4 different expression constructs. Got desired protein bands (9 kd) for two constructs in...
10 October 2015 9,695 15 View
I made a clone with my insert (230bp) and vector (5kb) which is c-terminally flag tagged. After making the constructs I have checked by sequencing twice! (it`s sure that there is not...
04 April 2015 5,282 17 View
