How to optic buffer conditions for Protein interaction by SEC?

Hello all, I am relatively new to the field of Protein purification and FPLC. So, forgive my naive questions. I want to study if protein A and protein B interacts by SEC. However, the protein A...

31 December 2019 7,410 3 View

Exact opposite result in cell cycle phase at different time point after overexpression?

Hello, I did a general comparison of cell-cycle analysis between my protein/CNT overexpressed HEK cell poupation. However, after 24hour of transfection, it shows a 10% increase in S-phase whereas...

01 February 2017 869 6 View

Best cell synchronization method after transfection?

Hi, I am working with a slow proliferative cell line (doubling time 18-20 hours) and I want to perform cell cycle analysis by FACS after transient transfection with my protein of interest. I did...

01 February 2017 6,004 3 View

Is HEK293 a good model for studying effects on cell-cycle analysis?

31 December 2016 9,182 4 View

What is the best/quick way to check ADP-ribosylation?

09 October 2016 369 4 View

Suggestions for measuring global translation rate apart from polysome profiling?

07 August 2016 8,607 2 View

CoIP and strange results?

Hello all, I am looking for interacting candidates for my protein and after doing IP followed by mass spec (found twice), I found protein X to be enriched almost 1:1 ratio to my bait protein....

07 August 2016 3,168 7 View

Troubleshooting a IP-Mass spec strategy ?

Hello all, I am working with a protein domain which hypothetically can go into nucleus (as it contains two NLS). I wanted to do IP-Mass spec to see what kind of proteins it is interacting with....

06 July 2016 9,241 4 View

What is ideal time point for MG132 treatment?

Hi, I am planning to treat my cells (transiently transfected and I generally process transfected cells after 48 hours of transfection) with MG132 for preventing degradation. From literature, 10 um...

01 February 2016 3,478 7 View

What is the best buffer for maintaining transient nuclear protein interaction?

Hello, I am trying to find interacting protein partners for a small nuclear intracellular domain (which is my protein of interest). My plan is to do IP with nuclear extract followed by Mass...

31 December 2015 5,134 3 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

What is the most Operating System (OS) which can be INSTALL on Tiny hard devices like Raspberry Pi (RPi) hardware ?

I'm targeting to deploy a mesh network and manually configure MANET routing protocols. I'm preparing scenarios, architectures, and hard devices needed to do that. Are there some step-by-step...

03 March 2021 1,931 5 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

Which of the Covid-19 Vaccines would elicit the best immunoprotection and why?

I want to know which of the available Covid-19 Vaccines would elicit the best immunological response and why

02 March 2021 1,388 3 View

No title

02 March 2021 3,060 3 View