Is it possible to quantify calcium carbonate from XRD data?

I have not done the analysis but planning to. I want to estimate CaCO3 in algal biomass.

03 April 2018 6,018 5 View

Anybody experienced in using MPN for enumeration of microalgae?

I would like to know how the positive tubes are scored in MPN for microalgae. Fluorometry or chlorophyll a or any other plating method ?

31 December 2016 5,603 4 View

Inverted fluorescent microscope on lease. Is this service available in India?

08 September 2016 6,891 1 View

Anybody know of a scientific supplier who can supply a basic model of 22 litre autoclave(pic attached)?

07 August 2016 1,898 3 View

Is it possible to increase concentration of a v/v solution without increasing volume? If so, how can this be achieved?

I am working on culturing algae with effluent. I want to scale up the effluent concentration from 10% to 100% in sea water keeping the total volume constant. Will it be possible?

03 April 2014 7,495 1 View

SEM and TEM facilities in and around Tamil Nadu, India?

Can you guys list the institutions which provide SEM and TEM service for biological specimens?

07 August 2013 4,790 15 View

Inverse peaks in HPLC

Hi I was separating carbohydrates by RP-HPLC. I used to get the solvent peak before the analytes and it was normal. All of a sudden, the solvent peak got inversed. I don't know why? Can anyone...

11 December 2011 469 11 View

Hydroxyapatite chromatography

In hydroxyapatite chromatography of proteins, is it necessary to bake the slurry overnight in an oven? Has anybody performed hydroxyapatite chromatography of proteins? If so please guide me....I...

10 November 2011 8,746 0 View

Sugar preparation for HPLC

Do anyone know a method for sugar extraction from cyanobacteria for HPLC analysis?

08 September 2011 2,944 11 View

CO2 calcualtion from Bicarbonate added

Hi all, Do anyone know how to calculate the amount of Co2(in percent or ppm) from added bicarbonate into a solution. Will be a big help.

06 July 2011 7,293 0 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View

Do you kow of any database containing data on retention time, log P and polarizability for a large number of molecules?

Hi, I am a chemistry undergraduate doing my dissertation project on HPLC. In my project, I need to predict the HPLC retention time of 20 aromatic molecules, the calibration set. To do this I will...

24 February 2021 2,602 1 View

Any easy method of analyzing vitamin D in foods by HPLC?

Looking for method of extracting and analyzing vitamin D in foods by HPLC

24 February 2021 7,524 7 View

What HPLC Marker(s) should be used to detect anthocyanins in a sample?

What HPLC Marker(s) or standard is used to detect and quantify anthocyanins such as Delphinidin, Cyanidin in a given sample of plant extract?

23 February 2021 1,832 4 View

HPLC baseline failure (ACN/Water)?

My baseline increases without any explanation, it is only fixed by changing the solution B, will it be the column? Reagents? Bomb? Detector? Solution A: H3PO4 0.01% IN WATER Solution B: H3PO4...

21 February 2021 5,656 3 View

Which vial septa the right one to be used for HPLC?

This is the unanswered question stored very long in my mind and till now I could not find a right answer. Google search educated me that the red surface is the PTFE material compatible to wider...

21 February 2021 546 3 View

What is the stability of 2 3-dihydroxyquinoxaline in acidic water?

I'm developing a UHPLC assay, and the fluorescent product is 2,3-dihydroxyquinoxaline. The reaction to produce this requires hydrochloric acid (about 1.2 M). Will storage in this acidic...

20 February 2021 5,820 4 View