In hydroxyapatite chromatography of proteins, is it necessary to bake the slurry overnight in an oven? Has anybody performed hydroxyapatite chromatography of proteins? If so please guide me....I want to do it.
Thank you.
I have not done the analysis but planning to. I want to estimate CaCO3 in algal biomass.
03 April 2018 6,137 5 View
I would like to know how the positive tubes are scored in MPN for microalgae. Fluorometry or chlorophyll a or any other plating method ?
31 December 2016 5,695 4 View
Do you know of any service providers or institutes renting or leasing out inverted fluorescent microscope for a month or so. I have seen some providrs in USA, but are there any in India? Kindly help.
08 September 2016 7,002 1 View
As I am new to Mumbai I don't know any suppliers. Kindly help me to identify scientific suppliers who can deliver goods without delay.Kindly share address or contact details. Thank you.
07 August 2016 2,013 3 View
I am working on culturing algae with effluent. I want to scale up the effluent concentration from 10% to 100% in sea water keeping the total volume constant. Will it be possible?
03 April 2014 7,612 1 View
Can you guys list the institutions which provide SEM and TEM service for biological specimens?
07 August 2013 4,954 15 View
Hi I was separating carbohydrates by RP-HPLC. I used to get the solvent peak before the analytes and it was normal. All of a sudden, the solvent peak got inversed. I don't know why? Can anyone...
11 December 2011 652 11 View
Do anyone know a method for sugar extraction from cyanobacteria for HPLC analysis?
08 September 2011 3,054 11 View
Anybody worked/working in cyanobacterial/microalgal sugar analysis by HPLC
08 September 2011 1,869 0 View
Hi all, Do anyone know how to calculate the amount of Co2(in percent or ppm) from added bicarbonate into a solution. Will be a big help.
06 July 2011 7,402 0 View
A crude extract of fungal culture using EtOH was subjected to column and TLC and partially purified compound was obtained. UV vis spectrum of the compound/s has max absorbance at 218nm. The...
11 August 2024 9,801 2 View
Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...
11 August 2024 8,141 3 View
Hello experts, Does anyone know any free software about retention index prediction ?
08 August 2024 7,403 2 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
Why is TLC not a form of partition chromatography and paper chromatography not a form of absorption chromatography?
03 August 2024 7,035 3 View
or we can collaborate
01 August 2024 6,705 2 View
I need something like Camag's VisionCat software for photodocumentation of TLCs. Could you recommend something?
31 July 2024 8,415 1 View
Hi everyone, I'd like to better separate two close peaks coming from a bioconversion sample, I'm currently using a C18 KromaPhase 4,6mm Ø.I. SCHARLAU, particle size (µm): 10, pore size (Å): 100,...
30 July 2024 435 3 View
I am working on algal extract to which gas chromatography (Not GC-MS) spectrum I want to discover. My question is can we identify specific compounds using retention time if I compared the RT with...
29 July 2024 8,034 4 View
I'm having problems with ion exchange chromatography. After applying my sample (a fluorescent protein), a preferential path inevitably forms. The matrix I use is DEAE-Sephacel. Could someone...
27 July 2024 6,605 0 View