Hi everyone,
I'm trying to grow some Streptomyces on agar overlaid with cellophane, but haven't had any luck - no growth with S. coelicolor. This seems to be a pretty common Streptomyces technique, so what am I doing wrong?
- Bought cellophane, cut and autoclaved it between wet filter paper layers
- Placed it onto the petri dish, pressed out bubbles and let it dry
- Inoculated with a spore suspension and mycelia
Does it need to be a specific type of cellophane? Thanks for any help!
Aliya Mirza
Was your plate incubated for the right amount of time? At the optimum temperature of your bacteria?
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Joshua Burns
Yep. S. coelicolor M145 at 30 degrees should show growth within a couple of days max on the agar I was using, but haven't had any growth.
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