Two weeks ago I prepared magnetic beads for immunoprecipitation but I didn't get enough lysate to use them all. Are the beads still good to use even after being stored at 4° in RIPA lysis buffer (with 0,4% Igepal)?
My apologies - I equilibrated them by washing them 3x in the RIPA buffer. The antibody (anti-HA and anti-FLAG) are already covalently bound to the beads from the manufacturer. The beads themselves are stored at 4°C in provided buffer so I suppose that they will not be affected, however the antibody might?
From my experience using 3X Flag tag magnetic beads from Thermofisher Scientific, I was able to recover the beads by eluting the proteins off and washing the beads in a glycine buffer. It makes it so you can reuse beads, but be careful because too harsh of conditions will make the beads unusable.
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