After DNA extraction using CTAB method the dna is diluted generally in nuclease free water or TE buffer. Of the two components ie., nuclease free water and TE buffer which is better to be diluted with extracted DNA and kept for further storage. I have observed both ways. which one would give a better shelf life and efficiency of DNA
TE buffer is better than Nuclease free water in storing DNA.
But the main question is what are you going to do with the DNA. As some process do prefer DNA buffered in molecular grade water. In this case,. you should not use TE buffer to store DNA
As already said, freshly prepared, filter sterilized, Tris EDTA buffer pH 7.5 is recommended to store DNA for long term use. Tris will maintain the pH and not allow acification which might lead to depurination and eventually broken DNA and as DNases tend to use divalent cations for their activity, EDTA will chelate the metal ions and prevent any degradation. But if you want to use the DNA for downstream processes like PCR or restriction digestion, EDTA might affect the efficiency. Depending on your requirement, you may choose your storage conditions.
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